[Histonet] Oil Red O question
John Kiernan
jkiernan <@t> uwo.ca
Thu Nov 11 00:28:01 CST 2004
Ask your client if fat can be washed out
by water? You and I know it can't.
Persuade your client to buy a textbook
and join the Histochemical Society ($50
per year, with a journal arriving every
month!)
John Kiernan
London, Canada.
------------------------------------
Andrea Grantham wrote:
>
> This may be a crazy question. I'm doing mouse livers for a lab here and
> they requested Oil Red O. Did the stain - looked great. They are asking if
> it was possible that some of the fat washed away during the washing steps.
> The experiment requires that they quantitate the fat found in these
> sections. Is this a possibility? Is there a better way to quantitate fat?
> For the record - my washing steps are done under a gentle stream of tap
> water running into a corner of the stain dish or coplin jar - not a full
> force gush of water from the faucet directly on the slides.
> Thanks.
> Andi Grantham
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
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