[Histonet] Oil Red O question

[Andrea Grantham]

This may be a crazy question. I'm doing mouse livers for a lab here and 
they requested Oil Red O. Did the stain - looked great. They are asking if 
it was possible that some of the fat washed away during the washing steps. 
The experiment requires that they quantitate the fat found in these 
sections. Is this a possibility? Is there a better way to quantitate fat?
For the record - my washing steps are done under a gentle stream of tap 
water running into a corner of the stain dish or coplin jar - not a full 
force gush of water from the faucet directly on the slides.
Thanks.
Andi Grantham
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: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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