[Histonet] NFB vs. Paraformaldehyde

[jo-ann-e.bader <@t> staff.mcgill.ca]

Good Morning Histonetters,

I would like to get an idea from those histotechs that work with mouse tissue if
they prefer NFB or Paraformaldehyde as a fixative for routine histology.

I work for a group of 6 researchers, most of who do mouse work.   Their students
do the necropsies, remove and block the tissue, make up their own
paraformaldehyde, fix and submit the cassettes to me from processing and
staining.

I would like everyone to change to NFB to standarize the fixation.  I can
guarentee that no one makes Paraformaldehyde the same.  Some make it the same
day, some the day before, 2-3 days before  etc.

I am meeting with the big bosses this afternoon and would like some ammunition
if possible.

Thanks for the assistance.

Jo-Ann