[Histonet] Immuno Artifact?

[LuAnn Anderson]

My guess is water in the toluene/xylene from the dehydration and clearing 
run down. He can test this by changing the alcohols and xylenes--removing 
the coverslips--taking slides back through xylenes, and alcohols to 95% and 
then re-running them through fresh alcohols and xylenes/toluenes and 
coverslipping. If it is water, it will disappear.
LuAnn

At 11:36 AM 11/8/04, Bryan Hewlett wrote:
>I agree, but it could also be water since the Becke lines do not appear
>quite wide enough for air.
>
>Bryan Hewlett, ART, MLT
>Consultant Technologist
>Quality Management Program-Laboratory Services
>Toronto, Ontario,
>Canada.
>
>----- Original Message -----
>From: "Marshall Terry Dr, Consultant Histopathologist"
><Terry.Marshall <@t> rothgen.nhs.uk>
>To: "jason m" <kosmicdog <@t> hotmail.com>; <histonet <@t> pathology.swmed.edu>
>Sent: Monday, November 08, 2004 12:12 PM
>Subject: RE: [Histonet] Immuno Artifact?
>
>
>That surely is entrapped air under the coverslip.
>
>Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
>  Consultant Pathologist
>  Rotherham General Hospital
>  South Yorkshire
>  England
>         terry.marshall <@t> rothgen.nhs.uk
>
>-----Original Message-----
>From: jason m [mailto:kosmicdog <@t> hotmail.com]
>Sent: 08 November 2004 16:47
>To: histonet <@t> pathology.swmed.edu
>Subject: [Histonet] Immuno Artifact?
>
>
>Can anyone identify the cause of this black circle artifact? It is small
>black circles that look sort of like air bubbles only much too small which
>have been turning up on my immuno's this week. I have changed all solutions
>but still have the problem. Our lab uses toluene instead of xylenes, I am
>wondering if that is the problem. Also I use Tween-20 in my citrate buffer
>and I wonder if that could be the problem. At any rate I will be checking
>both of these but if anyone can help It will be appreciated immensely. I
>have sent a photograph of the artifact to the the list serv image archive
>(see link below) under the filename "artifact.jpg".
>
>http://www.histonet.org/site_images_frame.asp
>
>Thanks again,
>Jason
>
>
>
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