[Histonet] Xylene substitute problems

[jo-ann-e.bader <@t> staff.mcgill.ca]

Good morning to all histonetters,

At around May of this year I had to change from xylene to a xylene substitute
and have been hhaving problems eversince.  I am processing all kinds of mouse
tissue and I find that the tissue seems to be softer, produces more wrinkles
and is generally more difficult to cut.  Currently I am using 3 baths at 1 hour
each.  I have been using diffrent times throughout the summer.  If anyone has
any pointers I would appreciate it.

Thanks

Jo-Ann