[Histonet] Antigen Retrieval w/ B&D Steamer.....

[hymclab]

Here is what we do--  I got this off the histonet about 1 1/2 years ago and
it works wonderful.

Turn on rice steamer and put in coplin jar or jars of retrieval solution
while running down slides (this is sufficient time to get the solution to
the right temp., approx. 90 degrees C)
Place solutions into retrival solution(being sure to place 7 slides in each
coplin jar even if you need to add blank slides for fillers) and set rice
steamer for 20 minutes.  After 20 minutes take out coplin jars and let cool
for 10 minutes.  Proceed with staining.

This is a great procedure and provides very consistent staining.

Hope this helps,
Dawn Schneider, HT(ASCP)
Howard Young Medical Center
Woodruff, WI

-----Original Message-----
From: Brandon Stokes [mailto:cuttingedgehistology <@t> yahoo.com] 
Sent: Friday, October 29, 2004 6:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antigen Retrieval w/ B&D Steamer.....


Hi fellow Histonetters........
 
I have a quick question for anyone out there that knows anything about using
a Black&Decker Steamer for Antigen Retrieval.  
 
How long do you pre-heat the solutions?
How long do you leave in the steamer?
How long do you cool down?
 
Those are a few of the questions that I have so far......If there is any
other helpful advice using this method it would be greatly appreciated.
 
Thanks,
 
Brandon Stokes, HT(ASCP)
Lab Manager
Cutting Edge Histology Services, LLC
Tigard, OR
503-443-2157

		
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