[Histonet] Counterstain to use with blue and red substrates

d.gregg <@t> juno.com d.gregg <@t> juno.com
Wed May 26 12:03:19 CDT 2004

Hi all,
I am using blue (NBT) and  red (AEC)  substrates in a double stain and
wondered if anyone has a recommendation of a nuclear counterstain that
may work with this. I could use metanil yellow a a cytoplasmic stain but
would prefer a primarily nuclear stain. I have used Methyl green but it
has covered some of the blue in the past. Thanks.

Douglas Gregg
Plum Island Animal Disease Center 
Greenport, NY

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