[Histonet] Embedding

[Jackie.O'Connor <@t> abbott.com]

I worked in a derm lab for a few years, and it seemed best to embed the 
skins on a slight angle to the mold, so that when you cut through the soft 
tissue first, your knife wasn't hitting a straight line of (sometimes) 
hard epidermis.  Also, if you cut through the epidermis first, there is a 
tendency to damage the knife edge, or drag hard particles through the soft 
tissue,  creating scratch artifacts in your section. 
Jackie O'




FreidaC <@t> aol.com
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/21/2004 08:46 AM

 
        To:     histonet <@t> lists.utsouthwestern.edu
        cc: 
        Subject:        [Histonet] Embedding


I have a question for all of the derm and hard tissue people out there. 
How 
do you embed the sections?  Do you cut the dense or hard tissue first or 
last 
- or do you embed at an angle?  One individual is questioning the answer 
given 
in the study guide and so far I have different answers from those I have 
consulted.

Thanks,

Freida Carson
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