[Histonet] Formalin fixation of cells for paraffinembedding?

[Joanne Mauger]

Marsha,
I'm not sure about thyroglobulin, but I know S-100 positivety diminishes or disappears after alcohol fixation. If its well fixed in NBF first, the following alcs don't seem to hurt.
Jo

>>> "Marsha Linville" <marshal <@t> dcla.com> 05/21/04 09:52AM >>>
Joanne,
Would one of the antigens that you speak of that doesn't like alcohol
fixation be Thyroglobulin?  

Marsha Linville
DCL Medical Laboratory
Indianapolis, IN  46268
(317) 874-1232

-----Original Message-----
From: Joanne Mauger [mailto:MAUGER <@t> email.chop.edu] 
Sent: Friday, May 21, 2004 6:52 AM
To: eca9 <@t> georgetown.edu; histonet <@t> lists.utsouthwestern.edu 
Cc: histonet <@t> lists.utsouthwestern.edu 
Subject: Re: [Histonet] Formalin fixation of cells for paraffin
embedding?


To Eva,
Wrap the pellet in lens paper,and put it in a cassette to fix and process
with your tissues. I would skip the alcohol fixation and spin the cells down
in NBF, then proceed with processing and embed as usual. Some antigens don't
like alcohol fix.
Jo

>>> "Eva C. Andersson" <eca9 <@t> georgetown.edu> 05/20/04 03:18PM >>>
Hi everyone,
My lab wants to paraffin embed some cells to be used as positive 
controls for immunohisochemistry staining.
We have a cell line growing but need a good protocol for fixing the 
cells with formalin. Once the cells have been fixed the pellet will be 
embedded in paraffin by our histopathology department.

Does anyone have a good and easy method for the fixation part?

We will deliver the pellet to the histopathology department in 70% ethanol.

Thanks in advance,

Eva Andersson
Research Assistant
Georgetown University



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet