[Histonet] spleen

[Petia P Stefanova]

Hi,


I have problems to cut mouse spleen. I have already optimized  my processing
protocol but I still encounter difficulties to get good quality of spleen
sections.
My cutting angle is good but when I section the tissue comes off the ribbon.
I tried to cool it, soak it but it didn't work.
My processing protocol is:
10%NBF - 24h
70% ETOH - 45min.
95% ETOH - 45min
95% ETOH - 45min
100%ETOH - 45min
100% ETOH - 45min
100% ETOH - 45min
Xylene  - 45min
Xylene  - 45min
Wax - 3x1h

Any suggestion would be much appreciated
Thanks
Petia