[Histonet] Fixation and Decalcification of Bone Marrow Bxs.

[Nader, Alexander]

> About 6 months ago, we switched to fixing our bone marrows
> in 10% NBF saturated with EDTA (this fixes and decals at the 
> same time)
> with beautiful results with our IHC and also ISH. Even though our
> pathologists are elated that the IHC and ISH are great, they are not
> completely happy with the morphology of the H&E slides. I was just
> wondering if anyone has any suggestions so we can accomplish 
> both great H&E morphology and great stains with IHC and ISH. 

What is your formula for the NBF-EDTA mixture? pH?

Alexander Nader MD
Vienna, Austria