[Histonet] Nuclear (DNA) Stain

[Smith, Allen]

Methyl green does not work after Bouin fixation.  Sometimes the Feulgen
reaction does.  If you have enough tissue, try several different hydrolysis
times, e.g., 0 min., 2 min., 4 min., 6 min.  
0.1% toluidine blue at pH 4.2 will usually stain RNA violet and DNA indigo,
even after Bouin fixation.
Kernechtrot, alum-brazilin, or Ehrlich's hematoxylin stain nuclei very well
after Bouin fixation, but they stain nucleoproteins rather than DNA as such.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon E
Willman
Sent: Wednesday, May 12, 2004 9:15 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Nuclear (DNA) Stain

Hi,
I was wondering what is the best  special stain used for staining
nucleus (DNA) in tissue that has been fixed in Bouin's.  Any
help would be most appreciated.

Thanks in advance!

Sharon Willman


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