[Histonet] Fixing frozen sections in 4% Paraformaldehyde.

[Gayle Callis]

We prefer to do a time study with 4% paraformaldehyde - cold at 4C.
2,4,6,8,10 min. and have done the same with cold 4C acetone.  Some antigens
can withstand longer fixation in PFA and/or acetone, while picky antigens
require shorter time. 

Frozen sections are air dried at RT, then immersed all at the same time,
with a section pulled at specific time point, rinsed with Dulbeccos PBS x 3. 

It is work, but in the long run has paid off rather than go back and adjust
fixation time.        

At 11:35 AM 5/12/2004 -0500, you wrote:
>Is it common to fix frozen sections of unfixed tissue with 4% PFA the same
way they can be fixed with acetone? I know it will definitely take longer
than aceton fixation, but has that been done? Thanks.
>
>Nidal E Muvarak
>Associate Research Specialist
>Vascular Tissue Biomechanics Laboratory
>Department of Biomedical Engineering
>University of Wisconsin-Madison
>1550 Engineering Dr.; Rm. 2158
>Madison, WI 53706-1609
>Lab: (608) 265-8921; Office: (608) 265-4205; 
>Home: (608) 256-7934; Cell: (608) 332-6068
>http://vtb.bme.wisc.edu
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)