Re: [Histonet] cryoprotection before MMA embedding at -20°C

[Myri37 <@t> aol.com]

I found this protocol in reference of a publication of Erben RG, 1997
it's about "embedding of bone in methylmetacrylate an improved method 
suitable for bone in histomorphometry, histochemitry, and immunohistochemitry"
embedding is done at -20°C that is why i think it needs a cryoprotection.
it's the first time a lost all tissue, and i dont understand why.
thank you
Myriam