[Histonet] Periodic acid versus chromic acid for GMS staining

[Kathleen Roberts]

Interesting, because Richard-Allan Scientific has a kit that apparently 
uses 1% periodic acid...
http://www.rallansci.com/histology/histology.aspx?id=61

Still scratching my head today,
Kathleen

Gayle Callis wrote:

>Caveat:  Periodic acid may not be the acceptable oxidizing agent for fungus
>staining, there is a publication on this by Freida Carson with Jerry
>Fredenburgh in J of Histotechnology a few years back.  Periodic acid is not
>strong enough to do the job i.e. oxidation on fungal components.  One can
>get false negative results.  A lot of people use this, but may not be aware
>of problems one can have doing GMS with periodic acid oxidation - it works
>fine for basement membrane staining in 2 um kidney sections i.e. Jones
>methenamine silver, but for accurate results, chromic acid may be the
>better choice for fungus work. There was a huge discussion on Histonet on
>this subject a few years back. 
>
>Also, you still have to collect the silver solution as hazardous waste so
>one more thing should not be too much of a problem. I do feel for those who
>have to pay for this collection/waste disposal, we are fortunate our
>university picks up that cost.    
>
> 
>
>At 03:03 PM 5/5/2004 -0500, you wrote:
>  
>
>>In an attempt to remove hazardous chromic acid from the lab, I vaguely
>>remember replacing the Chromic Acid with 5% Periodic acid instead for the
>>    
>>
>GMS.
>  
>
>>Has anyone else done this?  If my memory serves me correctly - it works
>>    
>>
>fine -
>  
>
>>but I am old  . . . . er.  (must be from all the teenagers at home).
>>
>>Jacqueline M. O'Connor HT(ASCP)
>>Abbott Laboratories
>>Global Pharmaceutical Research and Development
>>Discovery Chemotherapeutics
>>
>>    
>>
>
>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology 
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367 (lab with voice mail)
>406 994-4303 (FAX)
>
>
>
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>