[Histonet] Histobath or alternative?

[Laurie Colbert]

We have the Thermo Electron (Shandon) histobath.  We use it to freeze frozen sections and it works well for us.  I don't know how it would work for your applications.  I'm assuming the brains and spinal cords are fairly small, so it would probably be ok.  You cannot control the temp, but that does not affect what we do.  Good luck.

Laurie Colbert

-----Original Message-----
From: Rebecca Nishi [mailto:rnishi <@t> uci.edu]
Sent: Monday, May 03, 2004 6:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Histobath or alternative?


Does anyone currently use the histobath with isopentane, or a similar bath?

Currently we freeze rodent brain and spinal cords in isopentane cooled to
-65 (in a plastic beaker surrounded by dry ice). We would like to purchase a
bath to get rid of the need for dry ice, and to more easily control the
temperature.

I know that Thermo Electron (Shandon) currently makes a histobath, but I
understand that the temperature is not controllable? Is this true? Does
anyone know of any alternatives. We just need something for a benchtop,
nothing too large, and probably under 5K.

Thanks,

Rebecca Nishi
UC Irvine


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