[Histonet] Testes Bouin's fluid

[Craig Squire]

I work in a contract research lab and we keep almost all of our testes, and
sometimes epididymides, in 70% alcohol permanently.  We rinse with distilled
water and then store in 70%.  All of  the slides are then stained with H&E
and they turn out really nice.
When we place the tissue in 70% COH we do not trim the tissue right away, we
wait a few days.
Hope this helps.

Heather Squire
----- Original Message ----- 
From: "John Kiernan" <jkiernan <@t> uwo.ca>
To: <balajimr <@t> drreddys.com>
Cc: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, May 03, 2004 9:40 AM
Subject: Re: [Histonet] Testes Bouin's fluid


> It does not make sense to put specimens in neutral
> buffered formaldehyde after fixing them in Bouin's
> fluid. Bouin's is, for many purposes, a better
> fixative than NBF because it confers characteristic
> patterns of chromatin in nuclei of different cell
> types and it also permits brighter staining of
> collagen and cytoplasm with anionic dyes. Bouin's
> fluid extracts cytoplasmic RNA and it can also
> make DNA Schiff-positive (Feulgen hydrolysis), and
> can damage red blood cells.
>
> Staining by both haemalum and eosin is stronger
> after Bouin's than after NBF, and in many tissues
> the microanatomy, as seen in paraffin sections, is
> more lifelike. There is less differential shrinkage
> of cells, tubules etc than you see in paraffin
> sections of specimens fixed in neutral formaldehyde.
> -- 
> -------------------------------
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London,   Canada   N6A 5C1
>    kiernan[AT]uwo.ca
>    http://publish.uwo.ca/~jkiernan/
>    http://instruct.uwo.ca/anatomy/530/index.htm
> _______________________________
> balajimr <@t> drreddys.com wrote:
> >
> > Dear Histonetter,
> >
> > We have been preserving testes (of rats and mouse) in bouin's solution
and
> > after 24 hrs washing them with 70% alcohol and fixing it back in 10%NBF.
> > But staining later with H & E is not that great. Can anybody suggest me
> > the best protocol for fixing,processing and staining  of testes.
> >
> > Dr. Balaji
> > Dept. Pre clinical safety evaluation,
> > Discovery research,
> > Dr. Reddys Laboratories ltd. Bollaram Road,
> > Miyapur, Hyderabad, 500 049
> >  Andhra Pradseh, INDIA
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