[Histonet] RE: Histonet Digest, Vol 5, Issue 47
Rushing, Roxane
RRushing <@t> pathgroupla.com
Mon May 3 07:29:30 CDT 2004
Please send
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Subject: Histonet Digest, Vol 5, Issue 47
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Today's Topics:
1. Re: IHC camera and scope set up? (Robert Schoonhoven)
2. Re: anti-CD45 antibody for mouse parrafin sections (Gayle Callis)
3. PA Job Descriptions (Amy Self)
4. RE: PA Job Descriptions (Gary Gill)
5. re anti laminin Ab reagent (Carl)
6. Re: Finding Plain Slides (yangpw <@t> umich.edu)
7. grinding bone (Histology)
8. Microscope parts (Bill Blank)
9. NSH Veterinary, Industry and Research Committee webpage
update (Gayle Callis)
10. Re: Microscope parts (mari.ann.mailhiot <@t> leica-microsystems.com)
11. nail sectioning (Bliss, Mary E.)
12. grinding bone (Histology)
13. Arizona Society conference (Karen Lahti)
14. Re: water bath policy (KAELPERS <@t> aol.com)
15. Re: slide stainers - vendors (KAELPERS <@t> aol.com)
16. Tissue array (yan gao)
17. RE: quickest freezing method AND maintain morphology of mouse
brains for LCM then microarray??? (Kemlo)
18. RE: nail sectioning (Thom Jensen)
19. Re: nail sectioning (mark.lewis <@t> thermo.com)
20. RE: laminin (GUTIERREZ, JUAN)
21. Looking for ZAP-70and CD38 antibodies (Nava, Josefa)
22. Container (Rebecca Barnhart)
23. RE: Container (Gladney, Diane C Ms MACH)
24. RE: Job interview questions (Kari Bradshaw)
25. Contamination of processor alcohols (White, Lori)
26. RE: Container (Greer, Patricia)
27. RE: Zinc formalin and 10% NBF (Smith, Allen)
28. Re: thick sections in zebrafish
(Don.Birgerson <@t> leica-microsystems.com)
29. Re: Tissue array (Scott Mordue)
----------------------------------------------------------------------
Message: 1
Date: Thu, 29 Apr 2004 13:08:13 -0400
From: Robert Schoonhoven <rschoon <@t> email.unc.edu>
Subject: Re: [Histonet] IHC camera and scope set up?
To: jason madore <kosmicdog <@t> hotmail.com>
Cc: histonet <@t> pathology.swmed.edu
Message-ID: <4091367D.1040804 <@t> email.unc.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
This is a question with MANY answers and all of them have merit. For my
light microscope I have an Olympus BH-2 with a Qimaging Micropulisher
5.0camera. My inverted Flou. microscope is an Olympus IX 71 with the
Olympus MicroFire 5 MP camera. I also have it setup so that I can move
the MicroFire to a second (light) microscope. Both of these cameras are
5 megapixil cameras the MicroFire will take 16 bit images and the
Qimaging takes 12 bit images. In addition to the digital cameras ( well
actually I had it before the digitals') we have a dedicated Olympus
setup for traditional film. Ok, that tells you what I have but not the
really important stuff - like why??? I'll try to make this short but
there is a lot of information to take into consideration (which means
that I will skip over a few things).
First one needs to take into account the available funds and where to
allocate them within your proposed system, as well as whether to go
digital or stay with the traditional film type of system.
Funds - spend as much as possible on the optics of the microscope, the
best camera in the world will not correct for bad glass (optics). It
really is a matter of user prefferance as to what the make of the scope
is but do get the best objectives that your budget will allow for.
Film vs Digital - As good as digital is with today's technology (16 MP +
cameras are available), film still gives the best resolution. That
said.... it's pretty darn hard to tell the difference between the two
when looking at a printed micrograph in a publication. We've been
submitting digital micrographs for several years with no complaints.
Archived film will last for centuries , digital storage media does not.
Photomicroscopy using a film camera actually takes a fair amount of
skill and knowledge without even going into the darkroom part of it,
where as I can show a student how to take a digital image in less then
an hour and have them get good micrographs each time (I said good, not
great). It should also be noted that while the old saying is "that film
is cheap take lots of pictures", electrons are cheaper take more". The
preceding is true but................ the initial cost of a dedicated
digital camera, software and computer is MUCH higher than that of a
dedicated film camera. Another good point is that the computer is your
darkroom for digital images and there are incredible things that you
can do with programs like Adobe Photoshop.
I could go on for many pages regarding the other important factors like
camera bit depth (8, 12, & 16), image analysis printing, software, ad
nausium but I'm running out of time.
Bob,
Robert Schoonhoven,
UNC-CH
jason madore wrote:
> Can anyone recommend a reasonable camera/scope/computer setup for
> taking microphotographs of standard IHC slides and TMA slides?
>
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------------------------------
Message: 2
Date: Thu, 29 Apr 2004 11:44:33 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] anti-CD45 antibody for mouse parrafin sections
To: "m. van mkempen" <m.vankempen <@t> erasmusmc.nl>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20040429114433.00bc8cf8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"
I suggest you buy the rat antiMouse anti CD45 monoclonal, the one you are
attempting to work with detects CD45 in rat tissues. BD Pharmingen carries
the rat antiMouse. I am not sure if the OX-1 clone even cross reacts to
mouse antigens, plus you will have mouse to mouse problems. Any clone with
OX-1 is usually a rat clone.
At 04:14 PM 4/29/2004 +0200, you wrote:
>Hello,
>
>Are there any people who have experience with the following anti-CD45
>antibody: mouse monoclonal, clone OX-1 from BD #550566.
>
>I am trying to get this antibody to work on MOUSE spleen parrafin
>sections (It works in RAT cryosections). The tissue has been fixed in 4%
>paraformaldehyde during 24h at 4 C. After making the sections (5-6
>microns) and deparrafinizing I did the following steps:
>
>Endogenous peroxidase blocking with 3% H2O2 in methanol for 20 min
>Rinse with PBS
>HIER with a citrate buffer (pH 6,0 and microwave 450W, 9 min)
>Rinse with PBS
>Block with PBS 5%BSA (30 min, RT)
>Incubate with primairy antibody dilluted 1:50 in PBS 5%BSA at (O/N, 4 C)
>Rinse with PBS
>Incubate with rabbit anti-mouse immunoglbulins (DAKO #Z 0259) dilluted
>1:50 in PBS 5%BSA (30min, RT)
>Rinse with PBS
>Incubate with monoclonal mouse PAP (DAKO #P 0850) dilluted 1:50 in PBS
>5%BSA (30min, RT)
>Rinse with PBS
>
>Enzyme detection with DAB substrate (5-7 min)
>
>Afterwards I can not detect anything besides some background from the
>endogenous peroxidase. Should I try another antibody or can I improve my
>protocol?
>
>Best regards, Marion van Kempen (Erasmus MC, Rotterdam The Netherlands)
>
>
>
>
>
>
>
>--
>----------------------------
>*- mkempen -*
>MAILTO:m.vankempen <@t> erasmusmc.nl
>-----------------------------------------
>
>_______________________________________________
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>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 3
Date: Thu, 29 Apr 2004 13:51:35 -0400
From: "Amy Self" <ASelf <@t> gmhsc.com>
Subject: [Histonet] PA Job Descriptions
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<39836CD6DB61654E8F95A35898C921860A86CF <@t> exchange.gmhpost.com>
Content-Type: text/plain; charset="iso-8859-1"
Dear Histonetters,
I need your help again......... Does anyone have a "Pathologist
Assistant Job Description" that they would be willing to share with me.....
Thanks in advance....amy
Amy Self
Georgetown Hospital Systems
843-527-7179 (phone)
843-520-7882 (fax)
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------------------------------
Message: 4
Date: Thu, 29 Apr 2004 13:05:18 -0500
From: Gary Gill <garygill <@t> dcla.com>
Subject: RE: [Histonet] PA Job Descriptions
To: 'Amy Self' <ASelf <@t> gmhsc.com>, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<ABE9B3BDD0A58743A66AFDF0E33B383B01E6C17C <@t> HALIBUT.dcla.com>
Content-Type: text/plain
Go to:
http://www.pathologistsassistants.org/Public_content/General_info/what%20is.
htm
-----Original Message-----
From: Amy Self [mailto:ASelf <@t> gmhsc.com]
Sent: Thursday, April 29, 2004 12:52 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PA Job Descriptions
Dear Histonetters,
I need your help again......... Does anyone have a "Pathologist
Assistant Job Description" that they would be willing to share with me.....
Thanks in advance....amy
Amy Self
Georgetown Hospital Systems
843-527-7179 (phone)
843-520-7882 (fax)
NOTE:
The information contained in this message may be privileged, confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please notify
us immediately by replying to this message and deleting it from your
computer. Thank you. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Thu, 29 Apr 2004 20:08:35 +0100
From: "Carl" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] re anti laminin Ab reagent
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002301c42e1d$5f66abf0$82412cd9 <@t> home>
Content-Type: text/plain; charset="iso-8859-1"
Sigma L9393 polyclonal. For me, works on human, mouse, chick, with
proteolytic or M/W pretreatment ( I prefer M/W; less variable).
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------------------------------
Message: 6
Date: Thu, 29 Apr 2004 15:12:30 -0400
From: yangpw <@t> umich.edu
Subject: Re: [Histonet] Finding Plain Slides
To: histonet <@t> pathology.swmed.edu
Message-ID: <1083265950.4091539e2fb50 <@t> mail.umich.edu>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
Usually I use superfrost for slide-mounted sections. And for free floating
IHC
sections, I buy Gold Seal precleaned slide from Fishersci (cat # 3064) and
gel
coat them.
Pengwei Yang
University of Michigan
------------------------------
Message: 7
Date: Thu, 29 Apr 2004 13:09:34 -0700
From: "Histology" <histo <@t> compbio.com>
Subject: [Histonet] grinding bone
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000d01c42e25$e53f1200$fda55742 <@t> ca.sprintbbd.net>
Content-Type: text/plain; charset="iso-8859-1"
Does anybody have a procedure for grinding bone on a buehler ecomet-3.
Thank you
connie
------------------------------
Message: 8
Date: Thu, 29 Apr 2004 15:32:15 -0500
From: Bill Blank <bill501 <@t> mindspring.com>
Subject: [Histonet] Microscope parts
To: histonet <@t> pathology.swmed.edu
Message-ID: <p06010204bcb5957a9fd2@[63.153.12.28]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Does anyone know where I can get a halogen lamp socket for A Reichert
Microstar IV scope?
TIA,
Bill
--
_____________________________
Bill Blank
http://kernunnos.com (Celtic studies and numismatics)
OBOD's Message board: http://www.druidry.org/board
------------------------------
Message: 9
Date: Thu, 29 Apr 2004 15:41:44 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] NSH Veterinary, Industry and Research Committee
webpage update
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20040429154144.00bc6a30 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"
Dear All,
The National Society for Histotechnology, VIR committee has an updated their
webpage. Go to www.NSH.org and click on VIR.
Updates are:
Corrections to the Animal Processing Manual's first edition are posted for
those of you who purchased the manual from NSH.
Message from the new chairman of VIR Committee.
Contact information
Watch for VIR related Literature and Links and comments from chairman of
Hard Tissue Committee.
A call to all VIR histotechnologists to join the committee (membership form
available on the VIR page) and update member personal information.
Gayle Callis
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 10
Date: Thu, 29 Apr 2004 16:43:30 -0500
From: mari.ann.mailhiot <@t> leica-microsystems.com
Subject: Re: [Histonet] Microscope parts
To: Bill Blank <bill501 <@t> mindspring.com>
Cc: histonet <@t> pathology.swmed.edu
Message-ID:
<OF256B84D3.420E0B60-ON86256E85.00773403-86256E85.00773F23 <@t> e-leica.com>
Content-Type: text/plain; charset=us-ascii
Bill
Please contact Mark Streebel at 716 686 3166. He should be able to help
you.
Regards
Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com
Bill Blank
<bill501 <@t> mindspring.com> To:
histonet <@t> pathology.swmed.edu
Sent by: cc:
histonet-bounces <@t> lists.utsouth Subject:
[Histonet] Microscope parts
western.edu
04/29/2004 03:32 PM
Does anyone know where I can get a halogen lamp socket for A Reichert
Microstar IV scope?
TIA,
Bill
--
_____________________________
Bill Blank
http://kernunnos.com (Celtic studies and numismatics)
OBOD's Message board: http://www.druidry.org/board
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Thu, 29 Apr 2004 15:07:08 -0700
From: "Bliss, Mary E." <mary.bliss <@t> northwestpathology.com>
Subject: [Histonet] nail sectioning
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
<27066863AD02214B81FE49477F3E71AD0D6F2C3C <@t> hinet1.hinet.org>
Content-Type: text/plain; charset="us-ascii"
Hi to All,
Does anyone have a good procedure for fixing and cutting in good
fingernail and toenail sections? We are trying to come up with
something which will allow us to produce a good stainable slide with ALL
of the tissue still intact on it! I have heard about using
trichloroacetic acid but can't seem to find a procedure anywhere. Have
also heard about /tried Nair and soapy water. Does anyone have any
information to share?
Humbly yours,
Mary E. Bliss
Lead Histologist
Northwest Pathology, P.S.
3614 Meridian St. Suite 100
Bellingham, WA 98225
(360)734-2800 x601
(360)734-3818 FAX
------------------------------
Message: 12
Date: Thu, 29 Apr 2004 16:04:40 -0700
From: "Histology" <histo <@t> compbio.com>
Subject: [Histonet] grinding bone
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000d01c42e3e$5b558d80$fda55742 <@t> ca.sprintbbd.net>
Content-Type: text/plain; charset="iso-8859-1"
Sorry, I'am processing the bones and embedding in (MMA)
Methy methacrylate. thanks connie
------------------------------
Message: 13
Date: Thu, 29 Apr 2004 17:19:31 -0700
From: "Karen Lahti" <karen <@t> gateslinger.com>
Subject: [Histonet] Arizona Society conference
To: "histonet" <histonet <@t> pathology.swmed.edu>
Message-ID: <KIEIKBJOAEGPLHDFFKEFEEJICLAA.karen <@t> gateslinger.com>
Content-Type: text/plain; charset="iso-8859-1"
The Arizona Society for Histotechnology and Region VII conference is planned
for June 3-5, 2004 in Phoenix, Arizona. The complete booklet along with
registration and membership forms can be obtained from our website -
www.arizonahisto.org. We have many workshop planned to include PCR,
Forensic Anthropology, HT Readiness, QIHC, H&E's from A to Z, IHC, Chemical
Hazards, ISH, Yoga, Infectious Diseases and TEM/SEM. Please Do Not submit
funds online. The forms are available to be downloaded then printed off.
Please contact me if you have any problems or questions.
We are having a drawing for a free room for three nights for paid
registrants by May 10, 2004. So far, we have 22 vendors coming to support
our society.
The Best Western Grace Inn in Ahwatukee has free airport shuttle and is
providing great room rates - $59/night for a single up to $79/night for a
quad. Hospitality suites and one-bedroom suites are available.
www.graceinn.com for more information.
Thank You,
Karen Lahti
ASH President
------------------------------
Message: 14
Date: Thu, 29 Apr 2004 21:04:12 EDT
From: KAELPERS <@t> aol.com
Subject: Re: [Histonet] water bath policy
To: nlouisea <@t> gis.net, Histonet <@t> lists.utsouthwestern.edu
Message-ID: <78.55a0f4c3.2dc3000c <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
I have 2 references that mention cleaning the water bath, however the word
cross contamination is not mentioned. If I find something I will send it to
you
- Good Luck with your CAP inspection!
Theory and Practice of Histotechnology
Sheehan, Hrapchak, second edition - "Processing of Tissue", Chapter 3, p.83
important point number 9 in the Tissue-Handling Chart For Sectioning.
Theory and Practice of Histological Techniques
Bancroft, Gamblin, fifth edition - "Tissue Processing and Microtomy",
Chapter
6, p.97 last sentence under the subheading floating out sections.
lge
------------------------------
Message: 15
Date: Thu, 29 Apr 2004 21:14:05 EDT
From: KAELPERS <@t> aol.com
Subject: Re: [Histonet] slide stainers - vendors
To: Sue.Kapoor <@t> uhsi.org, histonet <@t> pathology.swmed.edu
Message-ID: <6b.283d2342.2dc3025d <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hacker Linear Stainers...you can load until your heart is content. The only
difficulty may be matching your current stain schedule to one that will be
able to work on the hacker system. There is a timer you set permanently on
the
instrument and each rack will stay in that solution for that set time then
prompt the instrument to lift and move to the next solution container. It
is a
great system, the only other factor is that it is a relatively long
instrument
and requires some space.
lge
------------------------------
Message: 16
Date: Thu, 29 Apr 2004 22:11:00 -0400
From: "yan gao" <gliuygao <@t> hotmail.com>
Subject: [Histonet] Tissue array
To: histonet <@t> pathology.swmed.edu
Message-ID: <BAY99-F49cLC1G7kU5t0001659d <@t> hotmail.com>
Content-Type: text/plain
Hi, Histonet.
I am interested to get a few tissue array for different human cancers
and cell array from human tumor cell lines. Any recommendation?
Yan Gao
Ph.D
Norvatis
_________________________________________________________________
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------------------------------
Message: 17
Date: Fri, 30 Apr 2004 08:48:42 +0100
From: "Kemlo" <kemlo <@t> tiscali.co.uk>
Subject: RE: [Histonet] quickest freezing method AND maintain
morphology of mouse brains for LCM then microarray???
To: "Karl Brand" <k.brand <@t> erasmusmc.nl>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <40800C1000030126 <@t> mk-cpfrontend-4.mail.uk.tiscali.com>
Content-Type: text/plain; charset="iso-8859-1"
London on a January day. Quickest way to freeze anyone's brain; luckily
these Southeners are immune!
__________________________________________________
Broadband from an unbeatable £15.99!
http://www.tiscali.co.uk/products/broadband/home.html?code=SM-NL-11AM
------------------------------
Message: 18
Date: Fri, 30 Apr 2004 10:14:30 +0000
From: "Thom Jensen" <tissuearray <@t> hotmail.com>
Subject: RE: [Histonet] nail sectioning
To: Histonet <@t> lists.utsouthwestern.edu,
mary.bliss <@t> northwestpathology.com
Message-ID: <BAY12-F440anlzmUR7B0001db97 <@t> hotmail.com>
Content-Type: text/plain
Have you tried 15% ammonia water. We have had success at softing the
nail before processing and after if needed for sectioning. I have
used this technique for horse hoff as well.
Thom
>From: "Bliss, Mary E." <mary.bliss <@t> northwestpathology.com>
>To: <histonet <@t> pathology.swmed.edu>
>Subject: [Histonet] nail sectioning
>Date: Thu, 29 Apr 2004 15:07:08 -0700
>
>Hi to All,
>
>
>
>Does anyone have a good procedure for fixing and cutting in good
>fingernail and toenail sections? We are trying to come up with
>something which will allow us to produce a good stainable slide with
ALL
>of the tissue still intact on it! I have heard about using
>trichloroacetic acid but can't seem to find a procedure
anywhere. Have
>also heard about /tried Nair and soapy water. Does anyone have any
>information to share?
>
>
>
>Humbly yours,
>
>
>
>Mary E. Bliss
>
>Lead Histologist
>
>Northwest Pathology, P.S.
>
>3614 Meridian St. Suite 100
>
>Bellingham, WA 98225
>
>(360)734-2800 x601
>
>(360)734-3818 FAX
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_________________________________________________________________
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------------------------------
Message: 19
Date: Fri, 30 Apr 2004 08:48:20 -0400
From: mark.lewis <@t> thermo.com
Subject: Re: [Histonet] nail sectioning
To: "Bliss, Mary E." <mary.bliss <@t> northwestpathology.com>
Cc: histonet-bounces <@t> lists.utsouthwestern.edu,
histonet <@t> pathology.swmed.edu
Message-ID:
<OFB59A5ADF.CF722517-ON85256E86.00462531-85256E86.0047ED3E <@t> shandon.com>
Content-Type: text/plain; charset=us-ascii
Mary,
Have you ever tried cutting Frozen sections of the unfixed toenail and
fingernail ? You may be surprised to find they cut quite easily as a
frozen section compared to a paraffin processed block.
Best regards,
Mark
Mark Lewis
Product Specialist
Anatomical Pathology
Clinical Diagnostics
Thermo Electron Corporation
(412) 747-4013
(412) 788-1097
E-mail: mark.lewis <@t> thermo.com
"Bliss, Mary E."
<mary.bliss <@t> northwestpathology To:
<histonet <@t> pathology.swmed.edu>
.com> cc:
Sent by: Subject:
[Histonet] nail sectioning
histonet-bounces <@t> lists.utsouth
western.edu
04/29/2004 06:07 PM
Hi to All,
Does anyone have a good procedure for fixing and cutting in good
fingernail and toenail sections? We are trying to come up with
something which will allow us to produce a good stainable slide with ALL
of the tissue still intact on it! I have heard about using
trichloroacetic acid but can't seem to find a procedure anywhere. Have
also heard about /tried Nair and soapy water. Does anyone have any
information to share?
Humbly yours,
Mary E. Bliss
Lead Histologist
Northwest Pathology, P.S.
3614 Meridian St. Suite 100
Bellingham, WA 98225
(360)734-2800 x601
(360)734-3818 FAX
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------------------------------
Message: 20
Date: Fri, 30 Apr 2004 08:45:50 -0500
From: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>
Subject: RE: [Histonet] laminin
To: "Megan Kear" <Megan.Kear <@t> hunter.health.nsw.gov.au>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A61F23E937E4DA488E0C0F60093843D901E4970D <@t> ccetxm030.echristus.net>
Content-Type: text/plain; charset="iso-8859-1"
Does it have to be polyclonal? We use DAKO's monoclonal with very good
results. (1:20, no pretreatment). They also have a polyclonal, but I have
not tried it. Good luck,
Juan
-----Original Message-----
From: Megan Kear [mailto:Megan.Kear <@t> hunter.health.nsw.gov.au]
Sent: Wednesday, April 28, 2004 11:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] laminin
Hi Histonetters
Anybody using a good laminin polyclonal antibody. To be used on
formalin fixed paraffin fixed embedded sections.
thank you for your help.
Megan Clarke
HAPS
IHC dept
Newcastle
Australia
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Message: 21
Date: Fri, 30 Apr 2004 09:22:17 -0500
From: "Nava, Josefa" <JosefaNava <@t> texashealth.org>
Subject: [Histonet] Looking for ZAP-70and CD38 antibodies
To: "'HISTONET <@t> PATHOLOGY.SWMED.EDU'" <HISTONET <@t> PATHOLOGY.SWMED.EDU>
Message-ID:
<7A5E02BB0E0A2E409F971D889B200C298A53DB <@t> phdex05.txhealth.org>
Content-Type: text/plain; charset="iso-8859-1"
Hello to everyone!
Can someone tell me where I can get ZAP -70 and CD38 antibodies tnat will
work well with paraffin sections and also with Ventana Benchmark/ XT
stainer.
I thank you very much.
Josie
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Message: 22
Date: Fri, 30 Apr 2004 10:28:35 -0400
From: "Rebecca Barnhart" <RBARNHART <@t> summithealth.org>
Subject: [Histonet] Container
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s0922a70.008 <@t> ch-groupie.summithealth.local>
Content-Type: text/plain; charset=US-ASCII
Our OR has asked that we get different containers for the larger
specimens. Our current container is the basic container with a snap
lid, they would like a screw top lid. The concern is if the container
is dropped it will leak with the containers we have now. The only
problem I am having is trying to find a gallon screw top container. Any
ideas? Again thank you in advance.
Becky
------------------------------
Message: 23
Date: Fri, 30 Apr 2004 10:44:50 -0400
From: "Gladney, Diane C Ms MACH" <Diane.Gladney <@t> se.amedd.army.mil>
Subject: RE: [Histonet] Container
To: 'Rebecca Barnhart' <RBARNHART <@t> summithealth.org>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <9D41AB7C56F8304F98537ABD87B249F66261EF <@t> DASMTHGBZ001>
Content-Type: text/plain; charset="iso-8859-1"
You may have a hard time finding gallon size specimen containers with screw
tops. Our OR has a large deep tray with a handle that they use to transport
specimens to the Histology Dept. The containers that they are now using have
lids that snap on snuggly and I sometimes have difficulty getting these lids
off. How do they transport the containers to your lab? I have found that
screw top containers will leak like crazy if the lids are not put on
straight. We have trouble from our clinics even with the small screw top
containers leaking because they won't take the time to put the lids on
straight.
Diane
Diane C. Gladney, HT (ASCP)
Histology /Cytology Supervisor
Moncrief Army Community Hospital
P.O. BOX 484
4500 Stuart Ave.
FT. Jackson, SC 29207
(803) 751-2530
DSN 734-2530
EMAIL: diane.gladney <@t> se.amedd.army.mil OR
dcgx1 <@t> aol.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Rebecca
Barnhart
Sent: Friday, April 30, 2004 10:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Container
Our OR has asked that we get different containers for the larger
specimens. Our current container is the basic container with a snap
lid, they would like a screw top lid. The concern is if the container
is dropped it will leak with the containers we have now. The only
problem I am having is trying to find a gallon screw top container. Any
ideas? Again thank you in advance.
Becky
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------------------------------
Message: 24
Date: Fri, 30 Apr 2004 07:49:24 -0700
From: "Kari Bradshaw" <kbradshaw <@t> lcpath.com>
Subject: RE: [Histonet] Job interview questions
To: "Richard Larson" <richalarson <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <JMELLDIIEJFPHMONOAAFIEGHCEAA.kbradshaw <@t> lcpath.com>
Content-Type: text/plain; charset="us-ascii"
Dear Histonetters,
Here is a list that I have developed over the last few years. I hope it's
helpful.
Kari Bradshaw, HT(ASCP)
Laboratory Manager
Lower Columbia Pathologists
1217 14th Ave
Longview, WA 98632
(360)425-5620
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Richard
Larson
Sent: Thursday, April 29, 2004 8:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Job interview questions
I have a job interview next week with a private lab as a histotech. I have
my ht certification plus four years of experience doing embedding, staining
and some microtomy. I have been out of the lab the past three years doing
other work but would now like to return to the histology profession. If
anyone has had a recent interview, I would be interested in learning what
sort of questions interviewers will likely ask, so that I can be more
prepared. Thanks for any replies.
---------------------------------
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------------------------------
Message: 25
Date: Fri, 30 Apr 2004 11:11:03 -0400
From: "White, Lori" <lwhite <@t> lakeridgehealth.on.ca>
Subject: [Histonet] Contamination of processor alcohols
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A21B79E1584CD7118CE800B0D0DF18DAC23B79 <@t> OSHBDC4>
Content-Type: text/plain
Hi,
We use an alcohol/xylene recycler and routinely test the alcohol for xylene
contamination before recycling. We have noticed a significant proportion of
our alcohols are contaminated when tested. Through process of elimination,
we have determined that our contamination is coming from the alcohols on the
processor. We replaced all of our alcohols with clean, non-recycled alcohol
and ran a cycle. The alcohols were tested the following morning and one of
the alcohols was contaminated. Has anyone had experience with this?
Thanks,
Lori
Lakeridge Health Corporation
Ontario, Canada
------------------------------
Message: 26
Date: Fri, 30 Apr 2004 11:26:09 -0400
From: "Greer, Patricia" <pwg1 <@t> cdc.gov>
Subject: RE: [Histonet] Container
To: "Rebecca Barnhart" <RBARNHART <@t> summithealth.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C45D67C <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
Becky,
Nalgene makes a 1 gallon wide-mouth container with screw top lid - we
often get specimens sent to us in these containers. I'm sure any of the
supply catalogs would have them. Actually I just looked at the Fisher
catalog and they have several sizes of wide mouth bottles listed. You
can go to their web site (www.fishersci.com) and search for bottles in
their general laboratory section.
Pat Greer
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road
Atlanta, GA 30333
Our OR has asked that we get different containers for the larger
specimens. Our current container is the basic container with a snap
lid, they would like a screw top lid. The concern is if the container
is dropped it will leak with the containers we have now. The only
problem I am having is trying to find a gallon screw top container. Any
ideas? Again thank you in advance.
Becky
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------------------------------
Message: 27
Date: Fri, 30 Apr 2004 11:49:33 -0400
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] Zinc formalin and 10% NBF
To: <kemlo <@t> tiscali.co.uk>
Cc: histonet <@t> pathology.swmed.edu
Message-ID:
<494304423C63E246A5CF87A3AEEB577011B5E7 <@t> bumail01.barrynet.barry.edu>
Content-Type: text/plain; charset="iso-8859-1"
It's not the periodic table, but the electromotive series. Ions of
elements at the top of the series will replace free elements (only on the
surface of any but the tiniest particles) lower in the series. The ion is
reduced to the free element and the lower down free element is oxidized to
the ion.
Zinc is pretty low in the electromotive series, so many ions can be
reduced by zinc metal, converting the zinc metal to zinc ions.
Unfortunately, the zinc in tissues is there as ions already.
Any metal low enough in the series to reduce zinc ions to zinc metal
would be such a good reducing agent that it would reduce water to hydrogen.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo
Sent: Tuesday, April 27, 2004 2:43 AM
To: jkiernan <@t> uwo.ca; Wendy England
Cc: histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] Zinc formalin and 10% NBF
Can't you test the residual fixative for the presence or absence of zinc?
My Chemistry is not what it was but can't you do something with an element
and the double decomposition of a salt? Something to do with an element
higher up or lower down the Periodic Table that displaces an element from
a salt that is the other way round, can't remember. But NBF doesn't contain
the heavy metal does it?
Why is it important to know? All I can remember is that zinc formalin made
the tissue brittle, or was that lead? The nuclear stain was rather deep
too. Hope that helps.
__________________________________________________
Broadband from an unbeatable £15.99!
http://www.tiscali.co.uk/products/broadband/home.html?code=SM-NL-11AM
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------------------------------
Message: 28
Date: Fri, 30 Apr 2004 11:21:14 -0500
From: Don.Birgerson <@t> leica-microsystems.com
Subject: Re: [Histonet] thick sections in zebrafish
To: "Eric Tytell" <tytell <@t> fas.harvard.edu>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF0FB11429.8A0FC56A-ON86256E86.005920CB-86256E86.0059BA78 <@t> e-leica.com>
Content-Type: text/plain; charset=us-ascii
Hi Eric,
I have read your question with some curiosity. If the JB4 supports
your specimen without distortion, why not use a motorized rotary microtome
to section? If you have any questions, please feel free to phone me.
Don Birgerson
Leica Microsystems
Technical Assistance Center
Don.Birgerson <@t> Leica-Microsystems.Com
1-800-248-0123 ext 5918 7:00 - 4:00 CDT
"Eric Tytell"
<tytell <@t> fas.harvard.edu> To:
<histonet <@t> lists.utsouthwestern.edu>
Sent by: cc:
histonet-bounces <@t> lists.utsouth Subject:
[Histonet] thick sections in zebrafish
western.edu
04/28/2004 02:40 PM
Hi all --
I'm trying to get thick (between 50 and 100 micron) transverse sections
of
adult zebrafish (15 to 20mm long). I'm concerned about the 3D structure of
the muscle tissue, so I'd like to have the specimens embedded in some
supporting medium. I've had good luck using plastic (JB-4) sectioned with
a
saw, but the trouble with that method is that I lose 300microns to the saw
each time I cut. I'd like to try embedding in a different medium, but I
haven't been able to find a protocol. Has anyone tried to do something
like
this?
I'm currently experimenting with paraffin embedding. Specifically, does
anyone have suggestions for how long and in what to decalcify? I currently
have both EDTA and Poly-NoCal available. Also, how long should I
infiltrate
in paraffin?
Alternatively, I've tried using a cryostat microtome, but the muscle tissue
seems to be damaged by the freezing or possibly by desiccation. Is it
necessary/possible to decalcify for frozen sections? Any suggestions for
avoiding freezing damage?
Also, does anyone know of a good polyclonal antibody for zebrafish skeletal
muscle? I'm not looking for anything too specific -- just enough to
distinguish skeletal muscle from bone and collagen under low magnification
confocal imaging.
I'd appreciate any suggestions. Thanks in advance,
Eric Tytell
------------------------------------------
Eric Tytell
Museum of Comparative Zoology Laboratories
Harvard University
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Message: 29
Date: Fri, 30 Apr 2004 09:44:16 -0700 (PDT)
From: Scott Mordue <i_stain <@t> yahoo.com>
Subject: Re: [Histonet] Tissue array
To: histonet <@t> pathology.swmed.edu
Message-ID: <20040430164416.15176.qmail <@t> web42002.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi Yan Gao,
I think you should contact Zymed. They have very high
quality Tissue Microarray, especially the human ones.
800-874-4494
Scott
CSU
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On
Behalf Of yan gao
Sent: Thursday, April 29, 2004 7:11 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Tissue array
Hi, Histonet.
I am interested to get a few tissue array for
different human cancers
and cell array from human tumor cell lines. Any
recommendation?
Yan Gao
Ph.D
Norvatis
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