[Histonet] Embedding in OCT and subsequent steps

[Starkus, Laurie]

Actually, that's not completely true.  Surgipath makes a mountant/clearing
agent in one called Clearium.  I use it instead of xylene or xylene
substitute.  Although, I guess in the strictest sense of the word, it is a
"substitute".

-----Original Message-----
From: Charles Scouten [mailto:cwscouten <@t> myneurolab.com]
Sent: Friday, April 30, 2004 2:52 PM
To: degaboh <@t> rice.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Embedding in OCT and subsequent steps


 Xylene infiltrates tissue, and prepares it for coverslipping (ie, with
Permount).  You still need xylene or a substitute if you plan to coverslip


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of ZP
Sent: Friday, April 30, 2004 12:08 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Embedding in OCT and subsequent steps

If I embed my sample in OCT, do I need to use xylene in later steps (i.e.
when staining or before mounting)?  I have a protocol that does that but I
thought xylene was really more for paraffin embedding.  Thanks!!

Zarana Patel
degaboh <@t> rice.edu


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