[Histonet] Testes Bouin's fluid

balajimr <@t> drreddys.com balajimr <@t> drreddys.com
Mon May 3 06:20:02 CDT 2004


Dear Histonetter,

We have been preserving testes (of rats and mouse) in bouin's solution and 
after 24 hrs washing them with 70% alcohol and fixing it back in 10%NBF. 
But staining later with H & E is not that great. Can anybody suggest me 
the best protocol for fixing,processing and staining  of testes.

Dr. Balaji
Dept. Pre clinical safety evaluation,
Discovery research, 
Dr. Reddys Laboratories ltd. Bollaram Road, 
Miyapur, Hyderabad, 500 049
 Andhra Pradseh, INDIA



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