[Histonet] levels
Julie.Sanders <@t> med.va.gov
Julie.Sanders <@t> med.va.gov
Mon Jun 28 07:06:17 CDT 2004
Our pathologists insist that we cut 4 levels on prostate biopsies. We stain
levels 1 and 4 and keep 2 and 3 unstained. I've tried to change this, we
hardly do anything with the unstained and end up tossing the slides. Very
expensive!
Julie Sanders
Supervisor, Anatomic Pathology
VAMC, Cincinnati
-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
[mailto:histonet-request <@t> lists.utsouthwestern.edu]
Sent: Saturday, June 26, 2004 1:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 7, Issue 37
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Today's Topics:
1. Re: levels on biopsies (Patti Loykasek)
2. Re: Steve Slap (Agripina Suarez)
3. RE: levels on biopsies (Laurie Colbert)
4. Steve Slap (DMBCMP <@t> aol.com)
5. Re: Steve Slap (Carolyn.Earley <@t> leica-microsystems.com)
6. Steve Slap (Jim Staruk)
7. Re: micowave QA?? (Dave Low)
8. Re: levels on biopsies (Gudrun Lang)
9. Re: cryostats (LISA MELLO)
10. antigen retreival (william hewett)
11. Steve Slapp/Jim Becker (Gldnsthisto <@t> aol.com)
12. New immunostainers, good IHC books? (Krat18 <@t> aol.com)
13. Re: levels on biopsies (Becky and Travis Barnhart)
14. Re: Steve Slap (Becky and Travis Barnhart)
15. Steve Slap: Funeral Arrangements (Traczyk7 <@t> aol.com)
16. Re: SPURR (Lesley Weston)
17. re: antigen retreival (katri <@t> cogeco.ca)
18. RE: Pollak's trichrome (Smith, Allen)
----------------------------------------------------------------------
Message: 1
Date: Fri, 25 Jun 2004 15:28:05 -0700
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: Re: [Histonet] levels on biopsies
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <BD01F505.5BFC%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"
Is there anyone out there who keeps unstain slides from each level? Just in
case you cut through an area of interest and need to do some more studies.
This is how I was trained, but that was long ago & far away!
Patti Loykasek
PhenoPath Laboratories
Seattle, WA
> We cut four levels (one section - trimming - next section etc.) on one
> slide. For prostata, mamma, kidney needle-biopsies we have special
> procedures to provide material for immunhistochemistry.
>
> Gudrun Lang, Linz, Austria
>
> ----- Original Message -----
> From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
> To: <histonet <@t> pathology.swmed.edu>
> Sent: Friday, June 25, 2004 8:00 PM
> Subject: [Histonet] levels on biopsies
>
>
> We have a lot of biopsy samples. We have always cut 3 levels. But,
> in an effort to save time and money (supplies), I would like to cut 2
> levels. What I want to know is how many levels everyone cuts on their
> biopsies? Does anyone just cut 2 levels?
> Thanks!
>
>
> Hazel Horn, HT/HTL (ASCP)
> Histology Supervisor
> Arkansas Children's Hospital
>
> Phone - 501.364.4240
> Fax - 501.364.3912
>
>
>
>
>
----------------------------------------------------------------------------
> --
> The information contained in this message may be privileged and
confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering
this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
notify
> us immediately by replying to the message and deleting it from your
> computer. Thank you.
>
============================================================================
> ==
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 2
Date: Fri, 25 Jun 2004 15:47:58 -0700
From: "Agripina Suarez" <ASuarez <@t> mrl.ubc.ca>
Subject: Re: [Histonet] Steve Slap
To: <Traczyk7 <@t> aol.com>
Cc: Histonet <@t> pathology.swmed.edu
Message-ID: <s0dc4949.070 <@t> mail.mrl.ubc.ca>
Content-Type: text/plain; charset=US-ASCII
I would like to send my condolences and sympathies to the family of
Steve Slap. I know him only through his contributions to the Histonet
and I am very grateful as a recipient of his knowledge that he
unselfishly shared with every one in the Histonet.
Thanks, Steve.
Agripina Suarez
Agripina C. Suarez
McDonald Research Laboratories
iCAPTURE Centre, St Paul's Hospital
1081 Burrard St, Vancouver
B.C., Canada V6Z 1Y6
Tel no. 604 682 2344 local 62703
Fax no. 604 806 8351
>>> <Traczyk7 <@t> aol.com> 6/25/2004 12:06:06 PM >>>
It is with much sadness that I am writing to report the passing of
Steve
Slap. He will truly be missed. A service is being held Monday, June
28th at the
Jewish Community Center in Amherst, MA. Steve's Mom requests that
flowers not
be sent. I asked about donations in Steve's name to a charity and she
had
not thought about that. Her and Lisa will talk about it. If I find out
more, I
will pass the information along.
For those of you who might want to attend the service, the center's
address
is 742 Main St. The telephone number is 413-256-0160. Mrs. Slap
thinks the
service will be at 11am but that's not definite. Check with the center
or keep
an eye on the HistoNet, when I find out for sure I will post it.
With best regards to all of you for keeping Steve & his family in your
prayers,
Dorothy
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Fri, 25 Jun 2004 16:04:28 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: RE: [Histonet] levels on biopsies
To: "Patti Loykasek" <ploykasek <@t> phenopath.com>, "Histonet (E-mail)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0BE6ADFAE4E7E04496BF21ABD346628001C5BF76 <@t> EXCHANGE1.huntingtonhospital.com>
Content-Type: text/plain; charset="iso-8859-1"
We cut three levels and three unstained slides on each prostate block.
Laurie Colbert
-----Original Message-----
From: Patti Loykasek [mailto:ploykasek <@t> phenopath.com]
Sent: Friday, June 25, 2004 3:28 PM
To: histonet
Subject: Re: [Histonet] levels on biopsies
Is there anyone out there who keeps unstain slides from each level? Just in
case you cut through an area of interest and need to do some more studies.
This is how I was trained, but that was long ago & far away!
Patti Loykasek
PhenoPath Laboratories
Seattle, WA
> We cut four levels (one section - trimming - next section etc.) on one
> slide. For prostata, mamma, kidney needle-biopsies we have special
> procedures to provide material for immunhistochemistry.
>
> Gudrun Lang, Linz, Austria
>
> ----- Original Message -----
> From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
> To: <histonet <@t> pathology.swmed.edu>
> Sent: Friday, June 25, 2004 8:00 PM
> Subject: [Histonet] levels on biopsies
>
>
> We have a lot of biopsy samples. We have always cut 3 levels. But,
> in an effort to save time and money (supplies), I would like to cut 2
> levels. What I want to know is how many levels everyone cuts on their
> biopsies? Does anyone just cut 2 levels?
> Thanks!
>
>
> Hazel Horn, HT/HTL (ASCP)
> Histology Supervisor
> Arkansas Children's Hospital
>
> Phone - 501.364.4240
> Fax - 501.364.3912
>
>
>
>
>
----------------------------------------------------------------------------
> --
> The information contained in this message may be privileged and
confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering
this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
notify
> us immediately by replying to the message and deleting it from your
> computer. Thank you.
>
============================================================================
> ==
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 4
Date: Fri, 25 Jun 2004 19:19:10 EDT
From: DMBCMP <@t> aol.com
Subject: [Histonet] Steve Slap
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <103.491b7d2e.2e0e0cee <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
To Steve's family.......
The Histology Department at Fresno Community Hospital in Fresno,
California, wish to express our sorrow at hearing of Steve's passing. We
were happy to have met him and were impressed by his knowledge. Our
hearts go out to you.
Dannie Blake, HT
------------------------------
Message: 5
Date: Fri, 25 Jun 2004 18:34:25 -0500
From: Carolyn.Earley <@t> leica-microsystems.com
Subject: Re: [Histonet] Steve Slap
To: HistoNet <@t> pathology.swmed.edu
Message-ID:
<OFD4C073B9.2D73C29D-ON86256EBE.008138CF-86256EBE.00817EAF <@t> leica-microsystem
s.com>
Content-Type: text/plain; charset=US-ASCII
Dorothy,
Thank you for sharing information about Steve to all of us on the histonet
during this final ordeal. Steve will be missed
by all who knew him and even those who didn't have the pleasure of meeting
him. Our community has indeed suffered
a great loss. Please let his family understand what he meant to all of
us.
Carolyn Earley
Traczyk7 <@t> aol.com
Sent by: To:
HistoNet <@t> pathology.swmed.edu
histonet-bounces <@t> lists.utsouth cc:
western.edu Subject:
[Histonet] Steve Slap
06/25/2004 02:06 PM
It is with much sadness that I am writing to report the passing of Steve
Slap. He will truly be missed. A service is being held Monday, June 28th
at the
Jewish Community Center in Amherst, MA. Steve's Mom requests that flowers
not
be sent. I asked about donations in Steve's name to a charity and she had
not thought about that. Her and Lisa will talk about it. If I find out
more, I
will pass the information along.
For those of you who might want to attend the service, the center's address
is 742 Main St. The telephone number is 413-256-0160. Mrs. Slap thinks
the
service will be at 11am but that's not definite. Check with the center or
keep
an eye on the HistoNet, when I find out for sure I will post it.
With best regards to all of you for keeping Steve & his family in your
prayers,
Dorothy
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 6
Date: Fri, 25 Jun 2004 19:45:41 -0400
From: "Jim Staruk" <jstaruk <@t> masshistology.com>
Subject: [Histonet] Steve Slap
To: <HistoNet <@t> pathology.swmed.edu>
Message-ID: <000c01c45b0e$8733df90$6501a8c0 <@t> yourw04gtxld67>
Content-Type: text/plain; charset="US-ASCII"
I've used this quote from time to time when I felt it appropriate. It's
Dick Solomon's eulogy to a fellow physicist from the old TV show "Third
Rock From The Sun". Tonight, it's appropriate:
"He was a man and a citizen of the world. As such, he was governed by
the laws of physics. What he is now is not or ever was really him, but
merely his shell that contained the life-force or energy that makes us
what we truly are. Science states that energy can neither be created or
destroyed. Energy moves at the speed of light. Einstein stated that as
anything approaches the speed of light its mass becomes infinite. So
you can see, Steve is not gone. He has become infinite and as such
surrounds us with his self and lives within our hearts both now and
forever.
Rest in peace Steve
Jim
________________________
James E. Staruk, HT(ASCP)
www.masshistology.com
------------------------------
Message: 7
Date: Sat, 26 Jun 2004 00:33:20 -0700 (PDT)
From: Dave Low <lowman034 <@t> yahoo.com>
Subject: Re: [Histonet] micowave QA??
To: KMB1904 <@t> aol.com, histonet <@t> pathology.swmed.edu
Message-ID: <20040626073320.92595.qmail <@t> web40904.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi Kathy,
This link has the title from Journal of
Histotechnology "Haven't You Calibrated Your Microwave
Oven Yet?"
http://joh.chnola-research.org/Archive/2002/25-1buesa.pdf
If this link fails, go to NSH.org then to Publications
to Journal of Histotechnology to Archives to Mar 2002.
At least the first page is available. You may have
to contact NSH on how to get a copy. Good Luck!
D. Low
Elmendorf Med Ctr, AK
--- KMB1904 <@t> aol.com wrote:
> Does anyone have a way of checking the working
> ability of their microwave??
> We just had JACHO...and the woman said we should be
> checking it for
> temperature consistency..and actually she thought
> checking it to see if it is losing
> microwave waves........Our biomed guys tell us that
> they are not responsible for
> microwaves..and I dont know where to start on this
> one....Any help is
> appreciated !! Thank you in advance!!
> Kathy Bowden HT
> Nanticoke Memorial Hospital
> Seaford, De 19973
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
__________________________________
Do you Yahoo!?
Yahoo! Mail - You care about security. So do we.
http://promotions.yahoo.com/new_mail
------------------------------
Message: 8
Date: Sat, 26 Jun 2004 09:39:17 +0200
From: "Gudrun Lang" <gudrun.lang <@t> aon.at>
Subject: Re: [Histonet] levels on biopsies
To: "Histonetliste" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <004401c45b50$afbc96e0$eeeea8c0 <@t> SERVER>
Content-Type: text/plain; charset="iso-8859-1"
We keep unstained slides until they are needed for immunhisto. Recently our
doctors decided, that the "old" unstained slides should be HE-stained to
keep the tissue-information. Formerly they were thrown in the waste.
Gudrun, Austria
----- Original Message -----
From: "Patti Loykasek" <ploykasek <@t> phenopath.com>
To: "histonet" <histonet <@t> pathology.swmed.edu>
Sent: Saturday, June 26, 2004 12:28 AM
Subject: Re: [Histonet] levels on biopsies
> Is there anyone out there who keeps unstain slides from each level? Just
in
> case you cut through an area of interest and need to do some more studies.
> This is how I was trained, but that was long ago & far away!
>
> Patti Loykasek
> PhenoPath Laboratories
> Seattle, WA
>
>
> > We cut four levels (one section - trimming - next section etc.) on one
> > slide. For prostata, mamma, kidney needle-biopsies we have special
> > procedures to provide material for immunhistochemistry.
> >
> > Gudrun Lang, Linz, Austria
> >
> > ----- Original Message -----
> > From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
> > To: <histonet <@t> pathology.swmed.edu>
> > Sent: Friday, June 25, 2004 8:00 PM
> > Subject: [Histonet] levels on biopsies
> >
> >
> > We have a lot of biopsy samples. We have always cut 3 levels. But,
> > in an effort to save time and money (supplies), I would like to cut 2
> > levels. What I want to know is how many levels everyone cuts on their
> > biopsies? Does anyone just cut 2 levels?
> > Thanks!
> >
> >
> > Hazel Horn, HT/HTL (ASCP)
> > Histology Supervisor
> > Arkansas Children's Hospital
> >
> > Phone - 501.364.4240
> > Fax - 501.364.3912
> >
> >
> >
> >
>
> --------------------------------------------------------------------------
--
> > --
> > The information contained in this message may be privileged and
confidential
> > and protected from disclosure. If the reader of this message is not the
> > intended recipient, or an employee or agent responsible for delivering
this
> > message to the intended recipient, you are hereby notified that any
> > dissemination, distribution or copying of this communication is strictly
> > prohibited. If you have received this communication in error, please
notify
> > us immediately by replying to the message and deleting it from your
> > computer. Thank you.
> >
============================================================================
> > ==
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 9
Date: Sat, 26 Jun 2004 05:45:17 -0400
From: "LISA MELLO" <steve8438 <@t> msn.com>
Subject: Re: [Histonet] cryostats
To: <histonet <@t> pathology.swmed.edu>, "Kathleen Spencer"
<kspencer <@t> utmem.edu>
Cc: Shannon Matta <smatta <@t> utmem.edu>
Message-ID: <BAY5-DAV30rjcxtniFK0000b62d <@t> hotmail.com>
Content-Type: text/plain; charset="iso-8859-1"
We have a Leica CM 1900 which is very user friendly and works extremely
well. It is great!!!
Steven Mello,HT(ASCP)
Anatomical Pathology Supervisor
Cape Cod Hospital
Hyannis, MA
----- Original Message -----
From: Kathleen Spencer
To: histonet <@t> pathology.swmed.edu
Cc: Shannon Matta
Sent: Friday, June 25, 2004 3:21 PM
Subject: [Histonet] cryostats
Hi everybody,
I am the person who months ago sent out the cry for help concerning
sections curling in the cryostat.
I was supposed to get back to you on what solved the problem. I wish I
could tell you, but the answer is that nothing has worked.
Our tissue cuts fine on another cryostat. The owner of the company has
apparently dismissed the issue and has not done anything about it,
although they did send their technician to try and help. After checking
everything out, she was at a complete loss. So now we have no choice but
to invest another large sum of money in a new cryostat.
Soooo, now I would like input from all of you as to which cryostat you
believe to be the best, and if you are planning to tell me it's Hacker
Bright, don't bother.
The Zeiss Microm cuts well, but the interior is too dark.
Any other suggestions would be greatly appreciated.
Thank you all in advance,
Kathleen Spencer, HT (ASCP)
Lab Manager/LCM Supervisor
UTHSC
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Sat, 26 Jun 2004 20:08:02 +1000
From: "william hewett" <wrhewett <@t> austarnet.com.au>
Subject: [Histonet] antigen retreival
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002001c45b65$78dcd760$9f98ddcb <@t> hewett>
Content-Type: text/plain; charset="iso-8859-1"
Hi All,
Has anyone a glorified electric pressure cooker called a
decloaking chamber in use in their lab.It is used to apply heat and pressure
in a controlled manner to tissue sections to restore antigens before
immunohistochemistry stains can commence.Its said to be superior to a
microwave pressure cooker by its manufacturers.
Bill Hewett
------------------------------
Message: 11
Date: Sat, 26 Jun 2004 08:33:52 EDT
From: Gldnsthisto <@t> aol.com
Subject: [Histonet] Steve Slapp/Jim Becker
To: histonet <@t> pathology.swmed.edu
Message-ID: <113.347a7a37.2e0ec730 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
I would like to request that the NSH Meeting in Toronto be dedicated to
those that we have lost this year in our Histotechnology Family.
Larry Fields(California)
------------------------------
Message: 12
Date: Sat, 26 Jun 2004 09:04:27 EDT
From: Krat18 <@t> aol.com
Subject: [Histonet] New immunostainers, good IHC books?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1e6.23af57c3.2e0ece5b <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
I asked yesterday for information about immunostainers that people really
like -- but what about immunostainers that people would NOT recommend, for
any
reason?
What seem to be the best books available for IHC staining? Our lab needs a
couple of good ones.
Karen_Raterman <@t> ssmhc.com
St. Mary's Health Center
6420 Clayton Road
St. Louis, MO 63117
Ph: 314-768-8423
FAX: 314-768-7145
------------------------------
Message: 13
Date: Sat, 26 Jun 2004 09:41:22 -0400
From: "Becky and Travis Barnhart" <barnhart717 <@t> comcast.net>
Subject: Re: [Histonet] levels on biopsies
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>,
<histonet <@t> pathology.swmed.edu>
Message-ID: <009501c45b83$48426250$ef285344 <@t> Tuckey>
Content-Type: text/plain; charset="iso-8859-1"
The majority of our cases are biopsies. We cut 2 levels for each. On each
slide, depending on the size of the tissue, we put 2 rows of sections. For
little specimens (colon polyps/bx, some cores, etc) we can usually get 8
sections on 1 slide.
Becky Barnhart
----- Original Message -----
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
To: <histonet <@t> pathology.swmed.edu>
Sent: Friday, June 25, 2004 2:00 PM
Subject: [Histonet] levels on biopsies
We have a lot of biopsy samples. We have always cut 3 levels. But,
in an effort to save time and money (supplies), I would like to cut 2
levels. What I want to know is how many levels everyone cuts on their
biopsies? Does anyone just cut 2 levels?
Thanks!
Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital
Phone - 501.364.4240
Fax - 501.364.3912
----------------------------------------------------------------------------
--
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please notify
us immediately by replying to the message and deleting it from your
computer. Thank you.
============================================================================
==
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Sat, 26 Jun 2004 09:50:54 -0400
From: "Becky and Travis Barnhart" <barnhart717 <@t> comcast.net>
Subject: Re: [Histonet] Steve Slap
To: <Traczyk7 <@t> aol.com>, <HistoNet <@t> pathology.swmed.edu>
Message-ID: <000901c45b84$9a2e41f0$ef285344 <@t> Tuckey>
Content-Type: text/plain; charset="iso-8859-1"
In only meet Steve once in a conference. It is a great loss to our
community. I have been following the thread since the beginning and hoping
for a better outcome. My thoughts and prayers are with his family during
this difficult time.
Becky
----- Original Message -----
From: <Traczyk7 <@t> aol.com>
To: <HistoNet <@t> pathology.swmed.edu>
Sent: Friday, June 25, 2004 3:06 PM
Subject: [Histonet] Steve Slap
> It is with much sadness that I am writing to report the passing of Steve
> Slap. He will truly be missed. A service is being held Monday, June 28th
at the
> Jewish Community Center in Amherst, MA. Steve's Mom requests that flowers
not
> be sent. I asked about donations in Steve's name to a charity and she had
> not thought about that. Her and Lisa will talk about it. If I find out
more, I
> will pass the information along.
> For those of you who might want to attend the service, the center's
address
> is 742 Main St. The telephone number is 413-256-0160. Mrs. Slap thinks
the
> service will be at 11am but that's not definite. Check with the center or
keep
> an eye on the HistoNet, when I find out for sure I will post it.
> With best regards to all of you for keeping Steve & his family in your
> prayers,
> Dorothy
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 15
Date: Sat, 26 Jun 2004 10:09:29 EDT
From: Traczyk7 <@t> aol.com
Subject: [Histonet] Steve Slap: Funeral Arrangements
To: HistoNet <@t> pathology.swmed.edu
Message-ID: <113.347b50c6.2e0edd99 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Steve's funeral is scheduled for 11am on Monday, June 28th at the Jewish
Community Center in Amherst. The address is 742 Main St. Amherst, MA 01002
T:
413-256-0160
Burial will take place immediately following at the JCA Cemetery, then
return
to the community center for a gathering of family and friends for reflection
and remembrance. No flowers, please. There have been several suggestions
as
to sending memorial gifts, certainly do whatever you feel appropriate. I
will
speak with Steve's Mom and Lisa regarding this matter and report back to the
histology community.
Dorothy
------------------------------
Message: 16
Date: Sat, 26 Jun 2004 07:21:06 -0700
From: Lesley Weston <lesley <@t> vancouverbc.net>
Subject: Re: [Histonet] SPURR
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BD02D462.7CA8%lesley <@t> vancouverbc.net>
Content-Type: text/plain; charset="US-ASCII"
How large are your blocks? If they contain implants, it sounds as if they
could be quite large, but Spurr's epoxy resin (I assume this is what you
mean) is usually used for blocks about 1mm cubed at most. You can do larger
blocks, but only by increasing the times and repetitions of all the
processing steps dramatically and using some kind of rotator, vacuum, and
any other tricks you might think of to force the materials into the tissue.
A lot of people use propylene oxide between the last alcohol and the first
resin, but I generally use graded mixes of alcohol/epoxy. I haven't heard of
anyone using xylene in this context. Hope this helps.
If you mean a different resin, then ignore everything I've said!
Lesley Weston.
on 24/06/2004 1:31 PM, Marks, Angela at Angela.Marks <@t> bsci.com wrote:
> Does anyone have any suggestions on working with SPURR? We are trying to
> embed animal vessels with implants. The block is polymerizing nicely but
> the tissue is gooey. I've tried a variety of different dehydration
> procedures (ethanol, acetone, xylene, amyl alcohol) and infiltration
> gradients.
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Sat, 26 Jun 2004 10:44:52 -0400
From: katri <@t> cogeco.ca
Subject: re: [Histonet] antigen retreival
To: "william hewett" <wrhewett <@t> austarnet.com.au>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <40dd8be4.36.6815.29573 <@t> cogeco.ca>
>Hi Bill,
We have three labs in this region doing immuno and all have finally
"graduated" to using decloakers. We have gone
through steamers, microwaves etc. and in my opinion the decloaker gives most
reliable results consistently. Microwaving
is efficient, but sometimes gave uneven results due to hot spots. I found
this to be true even using microwave pressure
cooker. Also it's great for doing QA, since it gives you temperature and
pressure readings, which then can be recorded.
I'm very happy with it...
Katri
Katri Tuomala
St.Joseph's Health Care
Hamilton, Ontario, Canada
> Hi All,
> Has anyone a glorified electric pressure cooker called a
decloaking chamber in use in their lab.It is used
> to apply heat and pressure in a controlled manner to tissue sections to
restore antigens before immunohistochemistry
> stains can commence.Its said to be superior to a microwave pressure cooker
by its manufacturers.
> Bill Hewett
> _______________________________________________
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------------------------------
Message: 18
Date: Sat, 26 Jun 2004 10:57:40 -0400
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] Pollak's trichrome
To: "Geoff McAuliffe" <mcauliff <@t> umdnj.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<4C051EAE581BB646BF53A749A73FBA2D1F3B58 <@t> exchsrv01.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
Gabe's trichrome is easier than Pollak's trichrome, does not require mercury
fixation, and almost always gives excellent results. It is given in
Kiernan's HISTOLOGIC AND HISTOCHEMICAL METHODS.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Geoff
McAuliffe
Sent: Thursday, June 24, 2004 6:04 PM
To: Bruce W Brodersen
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Pollak's trichrome
A friend of mine tried this many years ago and never could get good
colors. He went back to longer methods, but they worked.
Geoff
Bruce W Brodersen wrote:
>We've tried using the procedure listed on:
>http://members.pgonline.com/~bryand/StainsFile/stain/conektv/tri_pollak.htm
>
>We keep having collagen stain green rather than blue, as the procedure
>describes. Any suggestions as to what we are doing wrong?
>Thanks.
>
>Bruce W. Brodersen, DVM, PhD
>University of Nebraska Veterinary Diagnostic Center
>Fair St. & E. Campus Loop
>Lincoln, NE 68583-0907
>
>voice (402) 472-1434
>FAX (402 472-3094
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
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