[Histonet] Blocking in the CSA protocol

[Johnson, Teri]

Gayle Callis wrote: <snip> I have also heard the DAKO avidin/biotin kit
is made up with stronger concentration of avidin in order to accomodate
their CSA (tyramide amplification kit) use. <snip>
 
Kim et. al., An Improved Protocol of Biotinylated Tyramine-based
Immunohistochemistry Minimizing Nonspecific Background Staining
J. Histochem. Cytochem., Jan 2003; 51: 129 - 132.  They use 8% Tryptone
Casein Peptone (TCP) and a distilled water/Tween wash to reduce
background staining with their first incubation of SA-HRP.  Interesting
read.  Haven't tried it yet.
 
Regards,
 

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj <@t> stowers-institute.org