[Histonet] Pollak's trichrome

[Bryan Llewellyn]

You are not doing anything wrong.  It is an error on the page.  Since 
the method uses light green SF yellowish, it should say that collagen is 
expected to be green.  My apologies.

If you must have blue collagen, try substituting a blue acid dye for the 
light green.  Water blue (CI 42755) or methyl blue (CI 42780) would 
likely be satisfactory.  A bit of experimentation would be necessary to 
get the amounts correct, but it should work.

Bryan Llewellyn



Bruce W Brodersen wrote:
> 
> 
> 
> We've tried using the procedure listed on:
> http://members.pgonline.com/~bryand/StainsFile/stain/conektv/tri_pollak.htm
> 
> We keep having collagen stain green rather than blue, as the procedure
> describes.  Any suggestions as to what we are doing wrong?
> Thanks.
> 
> Bruce W. Brodersen, DVM, PhD
> University of Nebraska Veterinary Diagnostic Center
> Fair St. & E. Campus Loop
> Lincoln, NE  68583-0907
> 
> voice (402) 472-1434
> FAX (402 472-3094
> 
> 
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