[Histonet] phosphorylated antibodies

[Patti Loykasek]

Would you mind sharing some general guidelines with the list? I'm not
currently working with phosphorylated antibodies, but we have discussed
them. It may be one of those out there on the horizon that will eventually
impact more of us. 

Patti Loykasek
PhenoPath Laboratories




> We currently are doing several phosphorylated antibodies (pEGFR,
> p-4E-BP1(thr37/46, p-4E-BP1 (SER65), pP53, pP70S6Kinase, p107, pHE, pRb,
> and p-m-TOR) that are working very well. Sometimes they are a little
> more difficult to develop however I have found that the phosphorylated
> antibodies from Cell Signaling seem to work the best. If you are working
> with any of these, let me know and I'll share our protocol.
> 
> Charlene Henry, HT QIHC
> Histology/IHC Section Head
> Department of Pathology
> St. Jude Children's Research Hospital
> 332 North Lauderdale
> Memphis, TN  38105
> Phone: 901-495-3349
> FAX: 901-495-3100
> 
> 
> -----Original Message-----
> From: Patsy Ruegg [mailto:pruegg <@t> colobio.com]
> Sent: Wednesday, June 23, 2004 3:35 PM
> To: Histonet <@t> Pathology. Swmed. Edu
> Cc: Ihcrg <@t> Yahoogroups. Com
> Subject: [Histonet] phosphorylated antibodies
> 
> Is anyone working with phosphorylated antibodies?  Please share your
> experiences with us if you are.
> Thank you,
> Patsy
> 
> 
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