[Histonet] RE: Tie-2 antibody

[C.M. van der Loos]

Dear Kanwal,
We have good experience with the Chemicon rabbit anti-Tie-2 antibody AB3124. Dilution 1:1000 (= 0.2 ug/ml) overnight 4C. Detection with PowerVision-GAR/AP (ImmunoVision) and visualization with Permanent Red (DakoCyto).
Good luck,
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands


----- Original Message ----- 
>From  K Minhas <kminhas <@t> uoguelph.ca> 
Date  Tue, 22 Jun 2004 16:12:20 -0400 
To  histonet <@t> lists.utsouthwestern.edu 
Subject  [Histonet] Tie-2 antibody, 
Hi,
Does anyone have experience with this antibody for Westerns or IHC.
We have tried this antibody from several different sources and just
can't seem to get it to work well. For westerns, only the tissue lysates
from lung show some expression. 
Any suggestions would be greatly appreciated, especially for IHC.
Thanks
Kanwal Minhas