[Histonet] alcohol to xylene and back to alcohol again!?

[Weems, Joyce]

Hi Susie,

This works with breast and colons that are grossed in fresh at 7 pm for completion the next morning. I don't recall who first told us about this, but I think we heard on Histonet. It has worked well for us for several years. In addition, we still must squeeze the tissue between paper towels to help dehydrate it when the sections are too thick to process well. This processing schedule doesn't hurt this kind of tissue at all.  

Joyce

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Susie
Warwick
Sent: Monday, June 21, 2004 11:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] alcohol to xylene and back to alcohol again!?


I am a junior tech, slowly working my way through all the training.
mostly I'm at a microtome all day.
Anyway I've noticed that our processing schedule also has the backwards 
alcohol step (Exploding skin samples thread)

ie
alcohol
alcohol
xylene
alcohol <------
xylene
xylene
wax
wax

Now I dont have as much experiance as my supervisor, but it seems like a 
really weird processing schedule to me.
Does anyone else do this? Why is this step in there? I got told it was to 
help process fatty tissues?



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