[Histonet] Re: Neutral mucus stain

[Andrew Kennedy]

Hi Nancy,

 

Is this stuff still positive in a Diastase PAS? 

Might it be a glycoprotein or a glycolipid? 

A High Iron Diamine stain will distinguish glycoproteins from other
mucosubstances.

Try a Sudan fat stain or an Oil Red O for glycolipids

 

Regards

 

Andrew Kennedy

Senior Science Officer

Anatomical Pathology

Concord Repatriation General Hospital

Hospital Road
Concord NSW 2139

 

ph: +612 9767 6115

Fax +612 9767 8427

 

"corpora non agunt nisi fixata"

 

Message: 10

Date: Tue, 15 Jun 2004 21:27:13 -0700

From: staub <@t> gonzaga.edu

Subject: [Histonet] neutral mucus stain?

To: histonet <@t> lists.utsouthwestern.edu

Message-ID: <1087360033.40cfcc2153413 <@t> zagmail.gonzaga.edu>

Content-Type: text/plain; charset=ISO-8859-1

 

 

 

Does anyone know of a diagnostic stain for neutral mucus?  OR does anyone
know a good control tissue we could use?  We have identified a PAS +, alcian
blue negative, toluidine blue orthochromatic, carmine red slight positive
(and we know it's not glycogen) exocrine gland secretion (in an amphibian)
and think it is neutral mucus.  IS there anything else we can try that isn't
redundant?

 

thank you much.

 

Nancy Staub

Gonzaga University, Spokane, WA