[Histonet] Gms for Pneumo

[Cazares, Ruth]

We use 2 different controls, one for fungus and one for pneumo.  We always
leave the pneumo in the silver longer but sometimes it just gets brown-black
all over and the organisms don't stand out.  And then a couple days later we
do it (the same way) and it is absolutely beautiful (text book quality). I
can't figure out what makes it so variable.  Do the cut control slides get
old and not stain? I just don't know.

Ruth Cazares


-----Original Message-----
From: Jones, Sarah - RAS [mailto:sjones <@t> rallansci.com] 
Sent: Thursday, June 17, 2004 2:56 PM
To: 'Cazares, Ruth'
Subject: RE: [Histonet] Gms for Pneumo

Ruth,

Are you using a regular Fungus Control Slide or a Pneumocystis Control
Slide?  Pneumocystis always takes longer to stain, so a Pneumocystis Control
Slide should be used.  

Sarah

-----Original Message-----
From: Cazares, Ruth [mailto:RCazares <@t> schosp.org] 
Sent: Thursday, June 17, 2004 2:27 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Gms for Pneumo

In Response to Gayle's Inquiry as to how we do our stain,

 

We use the GMS procedure  in Freida Carson's book, but we use the microwave:

 

Heat the chromic acid @ 80% power for 50 seconds

Put the slides into the hot chromic and let sit for 10 minutes.

Wash and put in bisulfite for I minute and rinse.

Heat silver (25ml stock methenamine silver, 25ml DI water and 2 ml 5% Borax)

            37 seconds on high 

Place slides into hot silver and microwave on high for 10 seconds, let sit
for 3-5 minutes checking

            Under the microscope after 3 minutes.

Rinse, tone with 0.2% gold, then 2% thiosulfate  and counterstain with fast
green.

 

The fungus comes out very well each time, but the pneumo is very
inconsistent.

 

 

Ruth Cazares

 




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