[Histonet] Gms for Pneumo

[Cazares, Ruth]

In Response to Gayle's Inquiry as to how we do our stain,

 

We use the GMS procedure  in Freida Carson's book, but we use the microwave:

 

Heat the chromic acid @ 80% power for 50 seconds

Put the slides into the hot chromic and let sit for 10 minutes.

Wash and put in bisulfite for I minute and rinse.

Heat silver (25ml stock methenamine silver, 25ml DI water and 2 ml 5% Borax)

            37 seconds on high 

Place slides into hot silver and microwave on high for 10 seconds, let sit
for 3-5 minutes checking

            Under the microscope after 3 minutes.

Rinse, tone with 0.2% gold, then 2% thiosulfate  and counterstain with fast
green.

 

The fungus comes out very well each time, but the pneumo is very
inconsistent.

 

 

Ruth Cazares

 




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