[Histonet] Human or animal tissue for H&E control, a difference??

[Andrea Grantham]

I agree with Joe on the use of control tissue and might suggest using 
intestine, liver or maybe kidney as controls for H&E. You want to see nice 
crisp nuclear detail and I'd use something that shows the nuclei, probably 
not skin though.
Andi Grantham



At 10:03 AM 6/14/2004 -0500, you wrote:
>Ian:
>
>I would focus on using control tissue from the species that your research 
>utilizes.  If you are working with human tissue, then go with a human 
>control.  If mouse, use mouse control, etc.   As to the use of skin for 
>nuclear detail, I would be skeptical.  I will let others make a recommendation.
>
>Good luck,
>
>Joe Galbraith
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Bernard
>Ian R SSgt 59 CRES/MSROP
>Sent: Monday, June 14, 2004 9:10 AM
>To: Histonet (E-mail)
>Subject: [Histonet] Human or animal tissue for H&E control, a
>difference??
>Importance: High
>
>
>Would it make a difference as to what tissue (animal or human) I use as my
>H&E control?  We are not CAP regulated, as we are in research.  Hence we are
>not required to run controls but simply as good management practice and for
>QC.
>
>Is skin the best tissue control as to show nuclear detail?
>
>
>Thanks,
>
>SSgt B
>
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: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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