[Histonet] IHC protocols for Parvovirus Cat and dog

[Jan Shivers]

I use a monoclonal Ab to Canine Parvovirus on formalin-fixed,
paraffin-embedded tissue (it also cross-reacts with Feline Parvovirus).  The
supplier is Custom Monoclonals, Int.  It requires enzyme digestion for
antigen retrieval (proteinase K for 5 minutes used here); primary antibody
1:100 for 45 min.; EnVision+/Ms-HRP detection reagent for 30 min.; AEC for
15 minutes.

Custom Monoclonals info:  website - www.cmi.rcip.com;  email -
ckgrantcmi <@t> rcip.com; 813 Harbor Blvd., #284, W. Sacramento, CA 95691, USA;
telephone - 916-375-1139

Jan Shivers
Univ. of Minnesota Veterinary Diagnostic Lab

----- Original Message ----- 
From: "Lab BioVet AB" <biovetlab <@t> biovet.se>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, June 10, 2004 8:37 AM
Subject: [Histonet] IHC protocols for Parvovirus Cat and dog






Parvovirus to Canine/Feline

I need some help to set up an immunohistochemical protocols for
Canine/Feline Parvovirus.

 I have tried everything now and I on my way to give up, so I hope someone
can give me some tips and help.


I have a monoclonal antibody (host mouse) from Acris Antibodies.


I have try different pretreatment antigen-retrieval methods.


And different label systems, Envision + and StreptABComplex/HRP Duet kit
from DAKO.



I hope someone can help me





Regards



Biovet AB



Britt-Marie Smedberg



E-mail: biovetlab <@t> biovet.se
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