[Histonet] Microscopic Bubbles........by the hundreds & thousands

[Rosalba]

We use Dako CD3 on fixed tissue

 Original Message -----
From: "Tom Truscott" <ttruscot <@t> vetmed.wsu.edu>
To: "Bruce Abaloz" <brucea <@t> unimelb.edu.au>;
<histonet-bounces <@t> lists.utsouthwestern.edu>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, June 09, 2004 12:53 AM
Subject: RE: [Histonet] Microscopic Bubbles........by the hundreds &
thousands


Bruce, Maybe you will have to fix the plant in formalin first to stop
all natural processes such as gas formation. Good luck, Tom T. USDA
Pullman, WA.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bruce
Abaloz
Sent: Monday, June 07, 2004 5:49 PM
To: histonet-bounces <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microscopic Bubbles........by the hundreds &
thousands

Dear Histonetters,

We have  done the Oil Red O Charukian Method and Oil Red O technique
from Bancroft & Stevens, 3rd edition - both worked beautifully. Also
did Toluidine Blue stain combo with the Oil Red O & by itself - all
stunning results. Did the stains on cryo  -sections of a native
Australian plant to demonstrate that the 'grainy' bits on the
underside of the leaves were full of Lipids/fats.
  The PROBLEM we have encountered, is -  no matter what Aqueous
Mountant we try , our cryosections are covered in microscopic air
bubbles (have tried to date: BDH/ Aquamount; DAKO /Faramount &
Glycergel & Ultramount.....even plain ol' Glycerol, (coverslipping
from DH20/Deionized H20/Tap H2O/Hot H2O/air dried - no H20).......
ALL resulted with same problem.
Can someone PLEASE advise what we are doing wrong (???) OR suggest a
possible answer as we need these results to be photographed ASAP.
Cheers & THANKS in advance,

Bruce in Melbourne/OZ
--
BRUCE ABALOZ                           PH:61383446282
HISTOLOGIST                            FAX:61383447909
DEPT.of ZOOLOGY                        EMAIL: brucea <@t> unimelb.edu.au
THE UNIVERSITY Of MELBOURNE.
VICTORIA.AUSTRALIA 3010

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