[Histonet] Microscopic Bubbles........by the hundreds & thousands

[Tom Truscott]

Bruce, Maybe you will have to fix the plant in formalin first to stop
all natural processes such as gas formation. Good luck, Tom T. USDA
Pullman, WA.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bruce
Abaloz
Sent: Monday, June 07, 2004 5:49 PM
To: histonet-bounces <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microscopic Bubbles........by the hundreds &
thousands

Dear Histonetters,

We have  done the Oil Red O Charukian Method and Oil Red O technique 
from Bancroft & Stevens, 3rd edition - both worked beautifully. Also 
did Toluidine Blue stain combo with the Oil Red O & by itself - all 
stunning results. Did the stains on cryo  -sections of a native 
Australian plant to demonstrate that the 'grainy' bits on the 
underside of the leaves were full of Lipids/fats.
  The PROBLEM we have encountered, is -  no matter what Aqueous 
Mountant we try , our cryosections are covered in microscopic air 
bubbles (have tried to date: BDH/ Aquamount; DAKO /Faramount & 
Glycergel & Ultramount.....even plain ol' Glycerol, (coverslipping 
from DH20/Deionized H20/Tap H2O/Hot H2O/air dried - no H20)....... 
ALL resulted with same problem.
Can someone PLEASE advise what we are doing wrong (???) OR suggest a 
possible answer as we need these results to be photographed ASAP. 
Cheers & THANKS in advance,

Bruce in Melbourne/OZ
-- 
BRUCE ABALOZ                           PH:61383446282
HISTOLOGIST                            FAX:61383447909
DEPT.of ZOOLOGY                        EMAIL: brucea <@t> unimelb.edu.au
THE UNIVERSITY Of MELBOURNE.
VICTORIA.AUSTRALIA 3010          

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