[Histonet] Catecholamine-Fluorescence

Young Kwun kwuny <@t> email.cs.nsw.gov.au
Tue Jun 8 07:11:27 CDT 2004


Dear Martina,
I tried a different histochemical method for adrenergic nerves long time
ago. The introduction of Falk-Hillarp formaldehyde histofluorescence method
(Falk & Hillarp et al., Fluorescence of catecholamine and related compounds
condensed with formaldehyde, J. Histochem.Cytochem.10, 348-354, 1962) made
it possible to demonstrate peripheral stores of catecholamines.

However, as far as I know, a fluorescent technichque based on glyoxylic acid
has been proved to be superior to formaldehyde vapour technique. (Lindvall
et al., Histochemistry 39,197-227,1974; Furness & Costa, The use of
glyoxylic acid for the fluorescence histochemical demonstration of
peripheral stores of noradrenaline and 5-hydroxytryptamine in whole mounts,
Histochemistry 41,335-352, 1975).

I mainly used cryostat sections of fresh, ovine oesophageal muscle for my
study modified from Furness & Costa, but I remember some study using
freeze-dried and paraffin embedded tissue as well. I am not sure about
formalin fixed materials. I don't have any recent references. 

What about other enzymes for noradrenaline such as tyrosine hydroxylase and
dopamine beta-hydroxylase etc. Are there any reason not using
immunohistochemistry for adrenergic innervation?




Young Kwun
Senior Hospital Scientist
Dept. of Anatomical Pathology
Concord Hospital
Concord NSW 2139
Australia
Phone:61-2-9767 6075
Fax:61-2-9767 8427
Email:kwuny <@t> email.cs.nsw.gov.au
 

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
tinawegwerf-histosearch <@t> yahoo.de
Sent: Tuesday, 8 June 2004 6:14 PM
To: histonet
Subject: [Histonet] Catecholamin-Fluorescence

Dear Histonetters!
I would like to use the Formalin-induced Fluorescence to demonstrate
adrenergic nerve fibres in intestinal and urogenital tissues. Does anyone
have experience with that and would you have a protocol for that? Can I use
paraformaldehyde fixed tissue for the method or does it have to be fresh? I
would also like to try it on whole mount preparations of bladder wall.
Any help appreciated
Martina
Martina Pirker
Children's Research Centre
Our Ladies Hospital for Sick CHildren
Dublin
martina.pirker <@t> ucd.ie
 

		Mit schönen Grüßen von Yahoo! Mail.
Mit dem Yahoo! Messenger können Sie Freunde noch schneller erreichen!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


"This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Central Sydney Area Health Service."




More information about the Histonet mailing list