[Histonet] fibrin stain

[lpwenk <@t> sbcglobal.net]

What color is the mucin? If it's baby blue, the stain might be similar to a
Movat.

Otherwise, my guess for a fibrin stain would be MSB. It's like a Masson
trichrome (with fibrin red, collagen blue, muscle red, RBC red, and I
believe mucin bluish) with Martius yellow dye added to make the RBCs yellow,
instead of the usual red with most trichromes. That makes it easier to find
fibrin when it is in clot areas.

The original MSB used a double nuclear stain - Mayer hematoxylin followed by
celestin blue. And had somewhat unusual dyes - red being brilliant crystal
scarlet.

We've modified it at our hospital. We use Weigert hematoxylin only, instead
of double blue dyes. Nuclei show up better. Then we use our regular blue and
red dyes of our Masson trichrome. Along with the Bouin post-mordant. The
only difference is the additional step of Martius yellow.

Let me know if you need the MSB procedure.

Otherwise, I'm waiting on the edge of my seat, to find out what a WHO stain
is. (And, yes, I did get the Townsend comment.)

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Fred Underwood" <funderwood <@t> mcohio.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, June 04, 2004 2:28 PM
Subject: [Histonet] fibrin stain


> TGIF gang.
>
> I have a Doc trying to rule out an amniotic fluid embolism.  He is
> looking to demonstrate one or all of the following.  Fibrin, keratin,
> mucin.  One of the methods he mentioned was something called "the WHO"
> method, which utilizes celestine blue.  Of course very little procedural
> information was listed in the article.  I assume that Pete Townsend had
> nothing to do with it.  Any information you can offer would be greatly
> appreciated.  Thanks in advance.
>
> Fred Underwood
> Montgomery County Coroner's Office
> Dayton, OH
>
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