[Histonet] Xylene substitutes....again
convmcm <@t> cc.usu.edu
Thu Jun 3 12:24:30 CDT 2004
In response to your questions:
1) I used to use Histoclear.
2) It was satisfactory for most tissues, but it made brain, liver,
spleen etc. very friable and hard to cut. Also, I did not like the way
it performed in coverslipping my sections.
3)I'm not certain I know what you mean by having the same "power" as
xylene... I assume this means ability to clear tissues in processing and
dehydrated slides and in deparaffinizing sections prior to staining. I
thought it was mediocre as a deparaffinizer (required 10 minutes in 3
changes) and was not miscible with the mounting medium. It was supposed
to be compatible with xylene or toluene based mediums, but I did not
find this to be true. Maybe the manufacturer has a different concept of
"miscible" than mine. I dunno. Anyway... in the dehydration series on
the stainer, there seemed to be a lot of water absorption which made
changing each tub mandatory at the end of the day... and I live in a
very dry climate...
IMHO ... avoid histoclear. If you can use xylene, do it.
Oh, I forgot. That citrus odor was really nauseating.
Utah Veterinary Diagnostics Laboratory
Utah State University
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marceau,
Sent: Wednesday, June 02, 2004 7:32 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Xylene substitutes....again
I'd like to get further into a subject that was already debated on
1- Which one do you use or have you used before and have the results
satisfactory or not (maybe with a brief description of the specimen
2- Are these substitutes really better than Xylene,
3- Do they all have the same power than Xylene (I read about one that it
the same chemical power as Xylene compared to the other version....so
other does what exactly then?)
I am interested in changing our Xylene for a substitute but I need to
convince people on that.
531, boul. des Prairies
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