[Histonet] AE 13 stain?

Dana Settembre settembr <@t> umdnj.edu
Tue Jul 27 11:54:53 CDT 2004


Emily,
I first thought that you meant cytokeratin AE1 and AE3, a commonly used
cytokeratin antibody cocktial.
Just as Andi Kappeler mentioned. But now, Michael Fredrickson has some
specific info about pilar cytokeratin.  ?
Dana

>>> "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org> 7/27/2004
7:28:34 AM >>>
Does anyone know what AE-13 is? Is it an immunostain, and what is it
for?
Annette Featherstone HT/MLT

-----Original Message-----
From: Emily [mailto:emmie222 <@t> yahoo.com] 
Sent: Monday, July 26, 2004 13:47
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Securline markers


We have been having a major problem the Securline markers for about a
year -
they are pretty much dry when we get them. The company that
manufactures
them told me to add xylene to each marker. It works most of the time,
but it
takes up a lot of time to add the xylene. My feeling is that I
shouldn't
have to do that anyway - they should just make a marker that actually
works.
I have tried out many other pens/markers and have found none to be
satisfactory. The ink either fades too much during processing or just
totally washes off. Does anyone have any similar issues? Does anyone
have
any ideas or suggestions?
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