[Histonet] soften formalin fixed samples

[mari.ann.mailhiot <@t> leica-microsystems.com]

Jocelyne

You can soften your specimens a bit by soaking them on ice and water. You
will loose some of the specimen when you take your first sections. The ice
water will swell the tissue a bit. You could also soak the blocks on
glycerin, or liquid dish soap.

You may what to check your processing schedule of your specimens.  If you
have too many 100% alcohols this will definitely cause hard tissue. You may
be taking bound water out of the tissue. You may also check to see if you
have heat on during some of your processing steps. To much heat may also
dry your samples depending on the size of the samples.

Hope this helps.

Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com


                                                                                                                                                    
                      Leclerc Jocelyne                                                                                                              
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                      histonet-bounces <@t> lists.utsouth        Subject:  [Histonet] soften formalin fixed samples                                      
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                      07/27/2004 04:16 AM                                                                                                           
                                                                                                                                                    
                                                                                                                                                    




Good morning everybody!

I have a problem with my formalin fixed samples: they are just too hard.
Now
I wonder if there is a possibility to soften or "remoisture" them.
I would be very pleased if anyone of you felt able to help me.

Thank you and best regards,

Jocelyne
--
VetSuisse Fakultät
Veterinär-Anatomisches Institut
Winterthurerstr. 260
8057 Zürich


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