AW: [Histonet] Plasma membrane marker

[Peter Blaesse]

Hi Geoff,

my message was capable of being misunderstood. Sorry for that. I have an
antibody against my protein of interest (rabbit-anti-KCC2; KCC2 - a
potassium-chloride-cotransporter). I need a plasma membrane marker to do
co-stainings with anti-KCC2. So, the antibody against the marker protein
shouldn´t be a rabbit-ab.

peter

> -----Ursprüngliche Nachricht-----
> Von: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu]
> Gesendet: Dienstag, 20. Juli 2004 20:00
> An: Peter Blaesse
> Cc: histonet <@t> lists.utsouthwestern.edu
> Betreff: Re: [Histonet] Plasma membrane marker
>
>
> Hi Peter:
>
>     You will have to tell us what the "protein of interest" is before
> we, or anyone else, can answer your question.
> There are companies that will make antibodies to your protein, if
> nothing is commercially available. Such an antibody could be tagged with
> a marker for microscopy, if desired.
>     How do you know that Zamboni's is the best fixative if you are still
> looking for an antibody to your "protein of interest"?
>
> Geoff
>
> Peter Blaesse wrote:
>
> >I am looking for an antibody against a plasma membrane marker protein for
> >IHC (brainstem slices). The antibody should work after fixation with
> >Zamboni´s solution (2% PFA, 15% picrinic acid (aqueous solution)). This
> >fixation is optimal for the staining of my protein of interest.
> So, I do not
> >want to change the protocol. The antibody against my protein of
> interest is
> >from rabbit. Does anibody know an antibody that is suitable?
> >
> >Thanks for your help.
> >
> >peter
> >
> >
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> >
> >
> >
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
>