[Histonet] Plasma membrane marker

[Geoff McAuliffe]

Hi Peter:

    You will have to tell us what the "protein of interest" is before 
we, or anyone else, can answer your question.
There are companies that will make antibodies to your protein, if 
nothing is commercially available. Such an antibody could be tagged with 
a marker for microscopy, if desired.
    How do you know that Zamboni's is the best fixative if you are still 
looking for an antibody to your "protein of interest"?

Geoff

Peter Blaesse wrote:

>I am looking for an antibody against a plasma membrane marker protein for
>IHC (brainstem slices). The antibody should work after fixation with
>Zamboni´s solution (2% PFA, 15% picrinic acid (aqueous solution)). This
>fixation is optimal for the staining of my protein of interest. So, I do not
>want to change the protocol. The antibody against my protein of interest is
>from rabbit. Does anibody know an antibody that is suitable?
>
>Thanks for your help.
>
>peter
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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