[Histonet] OCT und freezing time

Ivo Schmidt ivoschi <@t> web.de
Fri Jul 16 10:03:10 CDT 2004


Hello experts,

I want to freezer my tissue quickly in OCT to preserve
tissue architecture and macromolecules (RNA, DNA, etc)
in isopentane at -80°C.
Does anybody know, how much longer you should immerse
an *OCT-embedded tissue* compared to a *naked tissue* (not OCT-embedded) ?

Thanx in advance.

Cordially,

Ivo.


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