[Histonet] Kidney Biopsies

[GUTIERREZ, JUAN]

If you are doing frozen sections for IF you do not want to use eosin, it lights up big time under fluorescent lighting.  We use to do our lung sections with and without Evan's Blue, that might help.  I think the blue was to get rid of background, so I think you could use it to mark your tissue.  Any other ideas out there on the net?

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
Christus Santa Rosa Hospital
333 N. Santa Rosa Ave.
San Antonio, TX  78207
(210)704-2533


-----Original Message-----
From: Featherstone, Annette [mailto:AFeatherstone <@t> KaleidaHealth.Org]
Sent: Friday, July 16, 2004 7:29 AM
To: 'SMITH,REBEKAH FELICIA'; Pathologyarts <@t> aol.com;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Kidney Biopsies


How is anyone freezing their kidney biopsies. Can we dab some eosin or
something on them to keep them visable?
Annette Featherstone
Supervisor, Kaleida Health Anatomic Path

-----Original Message-----
From: SMITH,REBEKAH FELICIA [mailto:rockbeki <@t> ufl.edu]
Sent: Thursday, July 15, 2004 22:47
To: Pathologyarts <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] thick and thin sections


I've occassionally gotten slightly assymetrical cuts due to the 
block moving in the microtome. I just usually take that to mean 
that I need push the block farther in and look at it from the side 
to make sure its straight before  cut again.

--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the 
stars
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"


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