[Histonet] RE: curl sections

Hernan Aldana haldana <@t> unimoron.edu.ar
Fri Jul 16 08:26:08 CDT 2004


For those trying to cut thick cryosections, it is not uncommon for them 
to curl. Just place them into your post -fix floating sections or PBS if
they are fixed, right when you cut them. They will flatten out. Mount 
onto charged or gelatin coated slides. Air-dry for a minutes ant then
run your Stains or immuno previous bath in distilled water. Per floating
protocol lose a lot of regents.. When done dip into alcohols, xylene,
coverslip.






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