[Histonet] Curled Brain Tissue

Rob Komorowski komo <@t> bu.edu
Thu Jul 15 12:09:26 CDT 2004


Not sure if anyone has any hints for this, but I have a few brains of
experimental rat brains that were sliced on a cryostat and hat curled, never
to flatten again. This has made getting an even stain on then very
difficult. Does anyone have any hints as to how to get them to unroll? I'm
staining them for c-Fos immuno and they were perfused with paraformaldehyde.
Thanks for any help!

Rob K





More information about the Histonet mailing list