[Histonet] Cutting Fat for IHC

[Krista J Gavel]

I am experiencing difficulties trying to section mouse abdominal adipose and mink adipose in the cryostat. The tissues were frozen at -80C in chunks. When attempting to make sections, the tissue sticks to itself, and clumps up. I have tried fixing in paraformaldehyde and using histofreeze, but the tissue is still too soft. The cryostat is at its lower limit, about -32C. Is there anything I can do?