[Histonet] RE: Antigen retrieval - Microwave problem

[Caroline Stott]

Hi Jamie,
We had big problems using breast tissue and sections boiling off the 
slide.  We ended up using superfrost plus slides, and bringing the citrate 
buffer to boil then leaving the tissue for 5 minutes.  We did this 3 times 
and the results were good.  The sections definitely held on better.  Also 
leaving the sections over night in a 37 or 56 degree incubator helped.
Good luck.
Caroline

Caroline Stott

Histology Service Unit
University of Otago
PO Box 913
Dunedin, New Zealand
Ph  (03) 479 7152
Fax (03) 479 7136