[Histonet] RE: Antigen retrieval - Microwave problem

[C.M. van der Loos]

Jamie,
The problem of sections coming off is mostly confined to tissue sections that contain a lot of fat. Leaving the slides in a stove at 37C for a couple of days sometimes helps. 
Recently we have been successful with an overnight antigen retrieval procedure at 70C (citrate 6.0 or Tris/EDTA 9.0 whatever you prefer). The sections (also the real fatty ones) nicely stay on the slides and antigen retrieval is just as good. In some cases even more effective.

Good luck,
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands 


----- Original Message ----- 
>From  Jaimie Hoh <jaimie_hk <@t> yahoo.co.uk> 
Date  Thu, 08 Jul 2004 15:36:11 +0100 (BST) 
To  histonet <@t> lists.utsouthwestern.edu 
Subject  [Histonet] Antigen retrieval - Microwave problem 
Hi Histonet,

I have a slight problem.

I am perfoming Antigen retrieval using microwave
boiling in Immunocytochemistry.

When I put the slides in the microwave and when the
citrate buffer boils, the specimen comes out from the
slide or it folds.

Does anyone have an alternative to performing Antigen
retrieval using microwave?

thanks,
Jaimie
Research Officer
Singapore Eye Research Institute