[Histonet] Cox-IHC in uterus

[Leclerc Jocelyne]

Good morning!
I have problems with performing COX-2-immunohistochemistry on bovine uterus.

I haven`t found an adequate antibody yet. Those that I tried showed
excessive unspecific bindings (Merck Frost) respectively didn`t bind at all
(BD Transduction labs, mouse monoclonal clone 33).
Additionally,  employing a Vectastain ABC Universal  Elite Kit (rabbit and
mouse IgG) I have severe problems with background, especially in the
perimetrium and connective tissue.
Seems like the secondary antibody cross-reacts with the bovine tissue
although I did peroxidase block (0.3% in methanol for 30 min), avidin-biotin
block (Vector), the normal serum which came along with the Vectastain kit
and sometimes tried an additional protein block (Dako).

I would be very happy if anyone of you out there could share the experience
with me and help with finding an antibody an eliminating the
"universal-kit-problems".
Thank you very much.

Jocelyne Leclerc


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Jocelyne Leclerc
Institute for Veterinary Anatomy
Vetsuisse Faculty, University of Zurich
Switzerland