[Histonet] RE:Recipe for Tris-EDTA, pH 9.0 for Antigen Retreival

[C.M. van der Loos]

Dear Robert,
Our recipe looks like this:

10 mM Tris / 1 mM EDTA buffer, pH 9.0
Dissolve 1.2 gram Tris in ± 950 ml distilled water.
Adjust pH to ± 9.5 with 2-4 drops of 1M HCl and add 0.37 gram EDTA (di-sodium salt).
Adjust pH to 9.0 with 1M HCl, and fill to 1 ltr with distilled water.
Store at 4°C.

Hope this works for you.

Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands


----- Original Message ----- 
>From  "Lott, Robert" <Robert.Lott <@t> bhsala.com> 
Date  Tue, 6 Jul 2004 11:48:47 -0500 
To  <histonet <@t> lists.utsouthwestern.edu> 
Subject  [Histonet] Recipe for Tris-EDTA, pH 9.0 for Antigen Retreival 
Anyone got a recipe for the solution above?  How do you dissolve the
EDTA in the solution?

Robert L. Lott, HTL(ASCP)
Manager, Anatomic Pathology
Baptist Health System
800 Montclair Road
Birmingham, AL   35213
205-592-5388  phone
205-592-5646  fax
robert.lott <@t> bhsala.com