[Histonet] Hydrophobic Pens

[SMITH,REBEKAH FELICIA]

I've been using Zymed pap pen (the thin kind) and usually it works 
fine. The only thing is if you're using a chromogen that can't be 
in xylene or alcohol (like AEC), if you put it on too thick, the 
pap pen marks might not rinse off that well.(I've been using AEC 
as of late,and I've found I can still use the pap pen, I just have 
to be careful of how much and rinse a lot with a couple changes of 
water after the AEC, or use a wash bottle to rinse.) If you're 
using DAB, the marks should disappear when you go up to xylene 
again.
On Thu Jul 01 15:09:06 EDT 2004, Nigel Woolf <nwoolf <@t> ucsd.edu> 
wrote:

> I have had a problem using Pap pens with immunofluorescent IHC: 
> ink leaches out and the secondary antibodies stick giving high 
> background. Any suggested pen for this application? Thanks! NW
> 
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--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the 
stars
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"


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